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The rapid expansion of synthetic biology is due to the design and
construction of synthetic gene networks that have opened many new
avenues in fundamental and applied research. Synthetic Gene
Networks: Methods and Protocols provides the necessary information
to design and construct synthetic gene networks in different host
backgrounds. Divided into four convenient sections, this volume
focuses on design concepts to devise synthetic gene networks and
how mathematical models can be applied to the predictable
engineering of desired network features. The volume continues by
highlighting the construction and validation of biologic tools,
describing strategies to optimize and streamline the host cell for
optimized network performance, and covering how optimally designed
gene networks can be implemented in a large variety of host cells
ranging from bacteria over yeast and insect cells to plant and
mammalian cell culture. Written in the successful Methods in
Molecular Biology (TM) series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
protocols, and notes on troubleshooting and avoiding known
pitfalls. Authoritative and easily accessible, Synthetic Gene
Networks: Methods and Protocols serves as an invaluable resource
for established biologists, engineers, and computer scientists or
novices just entering into the rapidly growing field of synthetic
biology
The completion of the Human Genome Project and the rapid progress
in cell bi- ogy and biochemical engineering, are major forces
driving the steady increase of approved biotech products,
especially biopharmaceuticals, in the market. Today mammalian cell
products ("products from cells"), primarily monoclonals, cytokines,
recombinant glycoproteins, and, increasingly, vaccines, dominate
the biopharmaceutical industry. Moreover, a small number of
products consisting of in vitro cultivated cells ("cells as
product") for regenerative medicine have also been introduced in
the market. Their efficient production requires comprehensive
knowledge of biological as well as biochemical mammalian cell
culture fundamentals (e.g., cell characteristics and metabolism,
cell line establishment, culture medium optimization) and related
engineering principles (e.g., bioreactor design, process scale-up
and optimization). In addition, new developments focusing on cell
line development, animal-free c- ture media, disposables and the
implications of changing processes (multi-purpo- facilities) have
to be taken into account. While a number of excellent books
treating the basic methods and applications of mammalian cell
culture technology have been published, only little attention has
been afforded to their engineering aspects. The aim of this book is
to make a contribution to closing this gap; it particularly focuses
on the interactions between biological and biochemical and
engineering principles in processes derived from cell cultures. It
is not intended to give a c- prehensive overview of the literature.
This has been done extensively elsewhere.
The completion of the Human Genome Project and the rapid progress
in cell bi- ogy and biochemical engineering, are major forces
driving the steady increase of approved biotech products,
especially biopharmaceuticals, in the market. Today mammalian cell
products ("products from cells"), primarily monoclonals, cytokines,
recombinant glycoproteins, and, increasingly, vaccines, dominate
the biopharmaceutical industry. Moreover, a small number of
products consisting of in vitro cultivated cells ("cells as
product") for regenerative medicine have also been introduced in
the market. Their efficient production requires comprehensive
knowledge of biological as well as biochemical mammalian cell
culture fundamentals (e.g., cell characteristics and metabolism,
cell line establishment, culture medium optimization) and related
engineering principles (e.g., bioreactor design, process scale-up
and optimization). In addition, new developments focusing on cell
line development, animal-free c- ture media, disposables and the
implications of changing processes (multi-purpo- facilities) have
to be taken into account. While a number of excellent books
treating the basic methods and applications of mammalian cell
culture technology have been published, only little attention has
been afforded to their engineering aspects. The aim of this book is
to make a contribution to closing this gap; it particularly focuses
on the interactions between biological and biochemical and
engineering principles in processes derived from cell cultures. It
is not intended to give a c- prehensive overview of the literature.
This has been done extensively elsewhere.
The rapid expansion of synthetic biology is due to the design and
construction of synthetic gene networks that have opened many new
avenues in fundamental and applied research. Synthetic Gene
Networks: Methods and Protocols provides the necessary information
to design and construct synthetic gene networks in different host
backgrounds. Divided into four convenient sections, this volume
focuses on design concepts to devise synthetic gene networks and
how mathematical models can be applied to the predictable
engineering of desired network features. The volume continues by
highlighting the construction and validation of biologic tools,
describing strategies to optimize and streamline the host cell for
optimized network performance, and covering how optimally designed
gene networks can be implemented in a large variety of host cells
ranging from bacteria over yeast and insect cells to plant and
mammalian cell culture. Written in the successful Methods in
Molecular Biology (TM) series format, chapters include
introductions to their respective topics, lists of the necessary
materials and reagents, step-by-step, readily reproducible
protocols, and notes on troubleshooting and avoiding known
pitfalls. Authoritative and easily accessible, Synthetic Gene
Networks: Methods and Protocols serves as an invaluable resource
for established biologists, engineers, and computer scientists or
novices just entering into the rapidly growing field of synthetic
biology
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