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Protein phosphorylation analysis is a central theme in current
analytical biochemistry, cell biology and systems biology. Due to
its versatility, specificity and sensitivity, mass spectrometry has
developed into a key technology in this field. A set of minor and
major instrumental innovations mean that mass spectrometers now
exhibit a level of performance, a stability of operation, a
relative ease of use, and productivity, which would once have been
hard to imagine. This book guides the reader through this prolific
field by presenting a collection of personal views and selected
examples which cover all the important principles with a focus on
electrospray ionization mass spectrometry. It covers:
phosphorylation analysis at the peptide, protein and proteome
level; manual and automated data evaluation; phosphopeptide
enrichment; quantitative aspects; element mass spectrometry;
individual analytical strategies, and hints to useful internet
resources. This book provides students, graduate students,
post-Docs and senior scientists from related areas with a better
understanding on molecular protein phosphorylation analysis. Its
highest aim is to strengthen the reader's ability to develop a
personal, well-founded opinion on original manuscripts published in
this field.
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