Biosensors for Single-Cell Analysis explores a wide range of biosensor technologies and their applications in single-cell characterization and analysis. Sections cover key biophysical and chemical single-cell properties that consider proteomic, metabolic, electrical, mechanical and optical properties. Each chapter features key definitions and case studies, providing detailed guidance for researchers who want to replicate covered solutions in their work. Tutorial sections, evaluations of the current state-of-the-field and future developments are also included. Microfluidic approaches to characterization, such as microfluidic impedance flow cytometry and microfluidic flow cytometry are considered alongside more conventional approaches, such as mass spectroscopy, fluorescent and mass flow cytometry. Additionally, key types of biosensors are covered, including atomic force microscopy, micropipette aspiration, optical tweezers, microfluidic hydrodynamic stretchers, microfluidic constriction channel and microfluidic optical stretchers.

The comparative analysis of historical linguistics focuses on reconstructing ancient patterns based on diachronic records and typological data from several languages or dialects in a language group. The ultimate aim of the comparative reconstruction which requires significant cross-linguistic observation and theoretical reasoning is to demonstrate the historical process of language changes. This book considers the diachronic development of both the Chinese language and the Naxi language, focusing particularly upon six contentious linguistic issues that are associated with various linguistic changes in most areas of the grammar of these languages, including phonological changes, semantic changes, syntactic changes, and contact-induced changes. These linguistic issues are: (1) tonal splits in proto-checked syllables and subgrouping of Loloish; (2) the semantic development of RETURN- in Chinese; (3) the semantic development of TAKE- in Chinese; (4) the development of agentive passive markers in certain dialects of Chinese; (5) definiteness and nominalization, relativization, and genitivization in Chinese; and (6) the development of nominalization, relativization, and genitivization in Naxi. This volume provides new methods and perspectives through which these issues can be analyzed and resolved on the basis of typological and diachronic evidence. It uses cross-linguistic data from Chinese and the Tibeto-Burman languages in order to reconstruct various diachronic developments in Chinese and Naxi.