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This volume provides a comprehensive and technical presentation of
numerous aspects of reproductive cell tissue cryopreservation, and
presents readers with current procedures and detailed discussions
of novel techniques and the latest innovations. The chapters in
this book are divided into five parts and cover subjects such as:
immature oocyte cryopreservation, human sperm vitrification and
slow-freezing, directional freezing of ovarian tissue, automated
vitrification systems, and detailed protocols on popular and
commercially available cryopreservation/vitrification systems.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
practical, Cryopreservation of Mammalian Gametes and Embryos:
Methods and Protocols includes chapters written by leading experts
in the field and is a valuable resource for anyone interested in
the field of cryopreservation.
This volume provides a comprehensive and technical presentation of
numerous aspects of reproductive cell tissue cryopreservation, and
presents readers with current procedures and detailed discussions
of novel techniques and the latest innovations. The chapters in
this book are divided into five parts and cover subjects such as:
immature oocyte cryopreservation, human sperm vitrification and
slow-freezing, directional freezing of ovarian tissue, automated
vitrification systems, and detailed protocols on popular and
commercially available cryopreservation/vitrification systems.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
practical, Cryopreservation of Mammalian Gametes and Embryos:
Methods and Protocols includes chapters written by leading experts
in the field and is a valuable resource for anyone interested in
the field of cryopreservation.
This concise, truncated version of Nagy, Varghese and Agarwal's
Practical Manual of In Vitro Fertilization is comprised of select
clinical chapters for a portable, affordable and up-to-date
resource. Clinical Embryology covers a variety of topics,
including: - Sperm processing methods - Embryo culture methods -
Insemination procedures - Embryo evaluation - Genetic testing of
embryos - Cryopreservation - Embryo transfer Also included are two
new chapters on predicting embryo developmental potential and
viability using automated time-lapse analysis (Eeva) and predicting
embryo implantation potential using Embryoscope. (TM) Practical for
both clinicians and researchers alike, Clinical Embryology brings
together all of the need-to-know information about these important
topics in reproductive medicine.
This concise, truncated version of Nagy, Varghese and Agarwal's
Practical Manual of In Vitro Fertilization is comprised of select
clinical chapters for a portable, affordable and up-to-date
resource. Gamete Assessment, Selection and Micromanipulation in ART
covers a variety of topics, including: - Human oocyte evaluation -
Sperm evaluation and selection - Micromanipulators and
micromanipulation - Biopsy procedures on oocytes and embryos -
Molecular insights in IVF Practical for both clinicians and
researchers alike, Gamete Assessment, Selection and
Micromanipulation in ART brings together all of the need-to-know
information about these important topics in reproductive medicine.
For around half of the couples who have trouble conceiving the
cause of infertility is sperm-related. Intracytoplasmic sperm
injection (ICSI) is the most common and successful treatment for
male infertility. Here, the pioneers for the technique, along with
authorities in the field, describe the underlying science of ICSI
and other micromanipulation techniques. Practical advice for
performing the techniques is covered in depth, including sperm
selection, laser-assisted ICSI, and the use of piezo in ICSI.
Examining the safety of ICSI in animal models as well as the impact
of ICSI on the health and well-being of the children conceived
through the procedure is discussed. This manual is an essential
resource for clinical embryologists and laboratory personnel
wishing to refine or develop techniques and improve outcomes.
The Practical Manual of In Vitro Fertilization: Advanced Methods
and Novel Devices is a unique, accessible title that provides a
complete review of the most well-established and current diagnostic
and treatment techniques comprising in vitro fertilization.
Throughout the chapters, a uniform structure is employed, including
a brief abstract, a keyword glossary, a step-by-step protocol of
the laboratory procedures, several pages of expert commentary, key
issues of clinical concern, and a list of references. The result is
a readily accessible, high quality reference guide for reproductive
endocrinologists, urologists, embryologists, biologists and
research scientists. The Manual also offers an excellent
description of novel procedures that will likely be employed in the
near future. An indispensable resource for physicians and basic
scientists, the Practical Manual of In Vitro Fertilization:
Advanced Methods and Novel Devices is an invaluable reference and
addition to the literature.
This concise, truncated version of Nagy, Varghese and Agarwal's
Practical Manual of In Vitro Fertilization is comprised of select
practical chapters for a portable, affordable and up-to-date
resource. Building and Managing an IVF Laboratory covers a variety
of topics, including: - Setting up and running an IVF laboratory -
IVF laboratory equipment and culture systems - Organization of the
IVF unit - Licensing and regulation in the ART laboratory - Quality
control and troubleshooting Practical for both clinicians and
researchers alike, Building and Managing an IVF Laboratory brings
together all of the need-to-know information about these important
topics in reproductive medicine.
Now in its revised and expanded second edition - including over 20
new chapters - this comprehensive textbook remains a unique and
accessible description of the current and developing diagnostic and
treatment techniques and technologies comprising in vitro
fertilization (IVF). Arranged thematically in sections, each
chapter covers a key topic in IVF in a sensible presentation. Parts
one and two describe the planning, design and organization of an
ART unit and IVF laboratory and equipment and systems,
respectively. The sections that follow provide detailed
descriptions of IVF techniques, embryo culture methods, sperm
processing and selection, insemination procedures,
micromanipulation, embryo evaluation, cryopreservation, and embryo
transfer. Concluding sections address issues of management and
regulation of ART labs across the globe, as well as special topics
and emerging techniques and devices. Chapter authors, all experts
in the field, contribute their expertise from around the world.
With the addition of learning key points and review questions at
the beginning and end of each chapter, this new edition of In Vitro
Fertilization is a readily accessible, high quality instructional
resource for reproductive medicine trainees at all levels.
Practicing reproductive endocrinologists, urologists, and
embryologists also will find value in the book, as will infertility
researchers.
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