This combined lecture and laboratory manual presents detailed protocols for the multi-step process involved in isolating a gene, cloning and characterizing it, expressing the encoded protein, purifying the protein, and characterizing rudimentary aspects of its basic physical properties. The manual includes 20 experiments designed to train students to prepare, manipulate and analyse plasmids, to produce fusion proteins in bacteria, and to purify these proteins based on unique chemical properties or substrate affinities. Maximizing its utility for both students and instructors, this unique manual precedes each lab experiment with the necessary background theory and principles, which culminate in detailed process rationale followed by the actual experimental technique. While many lab manuals list background references, this innovative book combines both theory and procedure into one manageable volume.

This is a reproduction of a book published before 1923. This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc. that were either part of the original artifact, or were introduced by the scanning process. We believe this work is culturally important, and despite the imperfections, have elected to bring it back into print as part of our continuing commitment to the preservation of printed works worldwide. We appreciate your understanding of the imperfections in the preservation process, and hope you enjoy this valuable book.

Montessori is a well known name, but who is the person behind it? This bilingual book introduces Italian educator Maria Montessori, her work, and her teaching method with a simple story, colorful illustrations, a short introduction, and additional interesting facts for curious minds. Two ready-to-use activities, to help readers review the book's content, are included at the end of the book. Language: Italian and English Large print Ages: 5+