Grapevine is one of the most widely cultivated plant species worldwide. With the publication of the grapevine genome sequence in 2007, a new horizon in grapevine research has unfolded. Thus, we felt that a new edition of Molecular Biology & Biotechnology of the Grapevine could expand on all the latest scientific developments. In this edition and with the aid of 73 scientists from 15 countries, ten chapters describe new aspects of Grapevine Molecular Physiology and Biotechnology and eleven chapters have been revised and updated.

This book is intended to be a reference book for researchers, scientists and biotechnological companies, who want to be updated in viticultural research, but also it can be used as a textbook for graduate and undergraduate students, who are interested in the Molecular Biology and Biotechnology of Plants with an emphasis on the Grapevine.

The book provides a reference for years to come, written by world-renowned expert investigators studying sex differences, the role of sex hormones, the systems biology of sex, and the genetic contribution of sex chromosomes to metabolic homeostasis and diseases. In this volume, leaders of the pharmaceutical industry present their views on sex-specific drug discovery. Many of the authors presented at the Keystone Symposium on "Sex and gender factors affecting metabolic homeostasis, diabetes and obesity" to be held in March 2017 in Lake Tahoe, CA. This book will generate new knowledge and ideas on the importance of gender biology and medicine from a molecular standpoint to the population level and to provide the methods to study them. It is intended to be a catalyst leading to gender-specific treatments of metabolic diseases. There are fundamental aspects of metabolic homeostasis that are regulated differently in males and females, and influence both the development of diabetes and obesity and the response to pharmacological intervention. Still, most preclinical researchers avoid studying female rodents due to the added complexity of research plans. The consequence is a generation of data that risks being relevant to only half of the population. This is a timely moment to publish a book on sex differences in diseases as NIH leadership has asked scientists to consider sex as a biological variable in preclinical research, to ensure that women get the same benefit of medical research as men.

This book provides a novel solution for existing challenges in wireless body sensor networks (WBAN) such as network lifetime, fault tolerant approaches, reliability, security, and privacy. The contributors first discuss emerging trends of WBAN in the present health care system. They then provide possible solutions to challenges inherent in WBANs. Finally, they discuss results in working environments. Topics include communication protocols of implanted, wearable and nano body sensor networks; energy harvesting methodologies and experimentation for WBAN; reliability analysis and fault tolerant architecture for WBAN; and handling network failure during critical duration. The contributors consist of researchers and practitioners in WBAN around the world.

Much has been written about the basic incompatibility of the dominant quantitative research model in psychotherapy and the qualitative preferences of the practitioner community providing psychotherapy. Researchers and clinicians are at odds over the most valuable type of knowledge needed: that emerging from quantitative, experimental research versus that from qualitative, case-based practice, respectively. Recently, a number of emerging research methods have attempted to bridge and integrate these two approaches. Case Studies within Psychotherapy Trials is one such effort and significantly furthers the synergy between them. The volume provides a comprehensive illustration of the "cases-within-trials" (CWT) model of research. Quantitative findings from four randomized clinical trials (RCT) are synthesized with qualitative and quantitative findings from systematic case studies of successful and unsuccessful clients representatively drawn from each RCT. The book opens with the history of dialectic and political controversy in psychotherapy research and recent initiatives to bridge the differing perspectives. The RCT and case study projects follow, each commented on by outside experts. In the final chapter the editors compare and contrast the separate projects and draw insightful, impactful conclusions. By bringing together quantitative, natural scientific perspectives on research and qualitative, interpretative understandings and strategies, the chapter authors demonstrate how practitioners can be meaningfully included in future psychotherapy research. This book will be of great interest to psychotherapy researchers and practitioners and those interested in research methods in the behavioral sciences more generally.

Aimed at both beginners and experienced researchers unfamiliar with the technique, this book provides a concise and easy-to-understand guide to using immunohistochemistry. It identifies the proper place and purpose of each component of immunohistochemistry, emphasizes the components that are of critical importance, and explains the logistics of experimental approaches. Understanding the principles of immunohistochemistry allows for the identification and localization of protein targets and is of crucial use in the following experiments: * producing transgenic animals * studying the effects of drug treatments * electrophysiological studies * animal surgery * isolating and differentiating stem cells * super-resolution microscopy Immunohistochemistry for the Non-Expert is aimed at a wide audience, including molecular biologists, pharmacologists, immunologists, chemists, cell biologists, physiologists, and researchers involved with animal anatomy studies.

Essentials from the series "Applied Scanning Probe Methods" are put together in the area of bio and biorelated systems. The contributions spread over all 13 volumes are now combined in this one volume. Thus, the reader will easily find a comprehensive overview of SPM applications for biosystems.

For scientists and professionals in academic and industry dealing with surface analytics of plant science, living cells, nanomedicine, cosmetics and biomimetics.

This book, which is the third volume of Biomedical translational research, focuses on the fundamental role of biomedical research in developing new medicinal products. It emphasizes the importance of understanding biological and pathophysiological mechanisms underlying the disease to discover and develop new biological agents. The book uniquely explores the genomic computational integrative approach for drug repositioning. Further, it discusses the health benefits of nutraceuticals and their application in human diseases. Further, the book comprehensively reviews different computational approaches that employ GWAS data to guide drug repositioning. Finally, it summarizes the major challenges in drug development and the strategies for the rational design of the next generation more effective but less toxic therapeutic agents.

This book describes the discovery of molecules from unexploited extreme marine environments, and presents new approaches in marine genomics. It combines the current state of knowledge in marine genomics and advanced natural products' chemistry to pursue the sustainable production of novel secondary metabolites (lead compounds), as well as pharmacologically active peptides/proteins, with antimicrobial, neuroprotective, anti-osteoporotic, anti-protozoan/anti-plasmodial, anti-ageing and immune-modulating effects. Further, it employs molecular-biology-based approaches and advanced chemical techniques to obtain and to select candidate compounds for pre-clinical and clinical studies.

This volume collects essays by the late bioethicist John D. Arras, best known for his many contributions to the methodology of bioethics. Always open-minded, Arras did not favor a single theory or view of method in bioethics, eschewing labels such as "casuist" or "pragmatist." He was conversant with the main philosophical methods that have dominated bioethics since the field's origin, including principlism, Gert's common morality, the "new casuistry", pragmatism, and others. Rather than defending any particular theory or method, though, Arras rigorously investigated those methods - and how they both expand and limit our field of vision. He sought, in the tradition of Kierkegaard, to make life "harder" for bioethics, by uncovering challenges to the field's analytical methods. His favorite mode of exploration and expression was the thoughtful essay. The essays collected here reveal him thinking through new problems and new possibilities, and they invariably yield fresh and valuable insights.

This book examines the current status of mHealth development, regulations and the social background in Japan, South Korea and China, comparing it to the situation in the United States and the European Union and consider solutions to issues surrounding mHealth. The recent progress in mobile technology, represented by smartphones and smart watches, has been remarkable. A service called mobile health (mHealth), which uses such mobile technology to manage health, is also becoming a reality. Although the accuracy of medical devices is not as accurate as those used in medicine, the biometric information such as heart rate and SpO2 can already be monitored over a long period of time. Although the technology is maturing to the point where it can be implemented in society, it remains an unapproved service of medical care in most countries. The development and social implementation of mHealth is most active in the US, but social implementation is gradually progressing in other countries as well. In this book, we will first discuss what kind of global and harmonized regulations are desirable by comparing the regulatory reforms necessary for social implementation of mHealth. In addition, mHealth raises privacy concerns in the US because the usual behavior and biometric information of subjects is utilized by private companies. In addition, it is important to note that the behavior and biometric information of subjects collected by smart devices is automatically analyzed by AI technology, mainly machine learning, which makes the analysis a black box.

The tumor microenvironment has become a very important and hot topic in cancer research within the past few years. The tumor microenvironment is defined as the normal cells, molecules, and blood vessels that surround and feed a tumor cell. As many scientists have realized, studying the tumor microenvironment has become critical to moving the field forward, since there are many players in a tumor's localized and surrounding area, which can significantly change cancer cell behavior. There is a dual relationship wherein the tumor can change its microenvironment and the microenvironment can affect how a tumor grows and spreads. Tumor Microenvironment in Cancer Progression and Cancer Therapy aims to shed light on the mechanisms, factors, and mediators that are involved in the cancer cell environment. Recent studies have demonstrated that in addition to promoting tumor progression and protecting tumor cells from the spontaneous immune-mediated rejection and different forms of cancer therapeutics, tumor microenvironment can also be a target and mediator of both standard and newly-emerging forms of cancer therapeutics. Thus, the dual role of the tumor microenvironment is the integral focus of the volume. The volume highlights the bi-directional interactions between tumor cells and non-malignant tumor component during tumor progression and treatment. It also focuses on the three groups of the reactive tumor component: stromal cells, blood vessels and the infiltrating immune cells. These three groups are discussed under the lens of their role in promoting tumor growth, shielding the tumor from rejection and from standard forms of cancer therapies. They are emerging as targets and mediators of standard and new forms of potential therapy.

Key Features: Provides botanical descriptions, distribution and pharmacological investigations of notable medicinal and herbal plants used to prevent or treat diabetes. Discusses phytochemical and polyherbal formulations for the management of diabetes and other related complications. Contains reports on antidiabetic plants and their potential uses in drug discovery based on their bioactive molecules.

This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.

This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Oral Biology: Molecular Techniques and Applications, Second Edition is a useful resource for oral biologists at all levels (students, early career researchers, and experienced veterans), and it provides a ready reference to new techniques and approaches that can be used to answer numerous specific scientific questions that may lead to a deeper understanding and treatments of oral diseases.

This volume takes an in-depth look at the potential pharmacological applications of 11 important antidiabetic plants, examining their antihyperglycemic, hypoglycemic, and anti-lipidemic properties along with current genome editing research perspectives. Plant natural products, or phytoconstituents, are promising candidates for antidiabetic pharmacological actions. The phytoconstituents, such as i' avonoids, terpenoids, saponins, carotenoids, alkaloids and glycosides, play vital roles in the current and future potent antidiabetic drug development programs Each chapter reviews a particular plant with antidiabetic properties, explaining the therapeutic aspects, its active antidiabetic compounds, and relevant genome editing technology. The specific plants discussed include Azadirachta indica (commonly known as neem, nimtree or Indian lilac), Gymnema sylvestre (commonly called gymnema, Australian cowplant, and Periploca of the woods), Syzygium cumini (commonly known as Malabar plum, Java plum, black plum, jamun or jambolana), Ceylon cinnamon (or true cinnamon, as opposed to cassia cinnamon), insulin plant (or Costus pictus), Trigonella foenum-graecum (better known as fenugreek), Mulberry, Nigella sativa L. (black caraway, also known as black cumin, nigella, kalojeera, kalonji or kalanji), Aegle marmelos (L.) (commonly known as bael (or bili or bhel), also Bengal quince, golden apple, Japanese bitter orange, stone apple or wood apple), Ficus benghalensis (the banyan, banyan fig and Indian banyan), and of course, garlic (Allium sativum). Antidiabetic Plants for Drug Discovery: Pharmacology, Secondary Metabolite Profiling, and Ingredients with Insulin Mimetic Activity will serve as a valuable source of information for students, drug researchers, medical practitioners, diabetic patients, and many others in the effort to gain understand of how these plant drug molecules can help fight diabetes.

Written by 30 authors from all over the world, this book provides a unique overview of exciting discoveries and surprising developments in human genetics over the last 50 years. The individual contributions, based on seven international workshops on the history of human genetics, cover a diverse range of topics, including the early years of the discipline, gene mapping and diagnostics. Further, they discuss the status quo of human genetics in different countries and highlight the value of genetic counseling as an important subfield of medical genetics.

Digital Transformation in Healthcare in Post-Covid19 Times discusses recent advances in patient care and offers critical comparative insights into their application across multiple domains in healthcare. By showcasing key problems, best practices and emerging challenges, the book offers a state-of-art review of opportunities and prospects in the process of delivering smart sustainable healthcare services. Topics discussed include healthcare challenges in the post-COVID-19 era, enabling technologies for digital transformation, value driven approaches to the delivery of patient centric top-quality health services, and analytics and enhanced decision making. In addition, the book updates knowledge on best practices for training towards digital transformation and sustainable health. This is a valuable resource for healthcare professionals, medical doctors, researchers, graduate students and members of the biomedical field who are interested in learning more about the use of emerging technologies in healthcare.

This book covers classic epidemiological designs that use a reference/control group, including case-control, case-cohort, nested case-control and variations of these designs, such as stratified and two-stage designs. It presents a unified view of these sampling designs as representations of an underlying cohort or target population of interest. This enables various extended designs to be introduced and analysed with a similar approach: extreme sampling on the outcome (extreme case-control design) or on the exposure (exposure-enriched, exposure-density, countermatched), designs that re-use prior controls and augmentation sampling designs. Further extensions exploit aggregate data for efficient cluster sampling, accommodate time-varying exposures and combine matched and unmatched controls. Self-controlled designs, including case-crossover, self-controlled case series and exposure-crossover, are also presented. The test-negative design for vaccine studies and the use of negative controls for bias assessment are introduced and discussed. This book is intended for graduate students in biostatistics, epidemiology and related disciplines, or for health researchers and data analysts interested in extending their knowledge of study design and data analysis skills. This book Bridges the gap between epidemiology and the more mathematically oriented biostatistics books. Assembles the wealth of epidemiological knowledge about observational study designs that is scattered over several decades of scientific publications. Illustrates the performance of methods in real research applications. Provides guidelines for implementation in standard software packages (Stata, R). Includes numerous exercises, covering simple mathematical proofs, consideration of proposed or published designs, and practical data analysis.

This volume explores experimental approaches used to study Duchenne muscular dystrophy (DMD), an X-linked degenerative skeletal muscle disease caused by mutations in the dystrophin gene. Including the latest progress and scientific achievements, the book covers recent discoveries achieved through in vivo gene editing which have proven to be promising in restoring dystrophin expression, at least in ameliorating skeletal muscle symptoms, and the contents focus on "Omics" techniques in gene expression, protein expression, miRNAs, and long non-coding RNA analysis, as well as experimental studies of the structural/functional changes affecting the skeletal and cardiac muscles and ongoing preclinical studies and clinical trials. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Duchenne Muscular Dystrophy: Methods and Protocols serves as a guide for researchers exploring the complicated nature of dystrophin in the hope of helping the victims of this disorder.

The go-to guide to evidence-based practice in nursing for more than a decade, Evidence-Based Practice in Nursing & Healthcare: A Guide to Best Practice, 5th Edition, presents the latest perspectives on research-backed nursing practice in an engaging, user-friendly approach that has made this the bestselling resource of its kind.    AJN award-winning authors Bernadette Melnyk and Ellen Fineout-Overholt combine straightforward, conversational storytelling, inspiring quotes, and engaging case studies to make evidence-based practice accessible for students at any level of familiarity. With real-world examples and meaningful strategies in every chapter, this revised and reimagined 5th Edition gives students the confidence to meet today’s clinical challenges and ensure the most effective patient outcomes for years to come.      New to this edition: NEW! Reimagined coverage and a new chapter on applying implementation science to clinical practice settings familiarize students with the latest evidence and emerging implementation and evaluation tools.  UPDATED! Content throughout empowers you to more effectively teach evidence-based practice principles in academic and clinical settings. UPDATED! Making EPB Real case studies reinforce clinical application through real-world examples. 

This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.

Counterfeit drugs continue to infiltrate the drug market in the United States, causing illness and death. This book addresses this issue and examines the recent trends in drug counterfeiting over the past 5-10 years. The text shows perspectives from crime lab and toxicology lab personnel and academic researchers, and includes topics such as a history of cases and issues with counterfeit drugs, trends observed in forensic labs, instrumental methods and approaches used in detecting counterfeit medicines, and policy approaches for controlling counterfeit drugs. There is a focus on ways to reduce counterfeit drugs in the market, to help improve the health and safety of people all over the world. Features : Focuses on recent (5-10) year trends in counterfeit drugs and analysis. Shows perspectives from crime lab and toxicology lab personnel and academic researchers. Focuses on drugs seized by law enforcement and approaches to reducing counterfeit medicine in the market. Discusses the detection and analysis of counterfeit drugs, and appropriate tools for combating this issue. Emphasizes the global impact of illegal medicines.

This book gives an introduction to the highly interdisciplinary field of biomaterials. It concisely summarizes properties, synthesis and modification of materials such as metals, ceramics, polymers or composites. Characterization, in vitro and in vivo testing as well as a selection of various applications are also part of this inevitable guide.

The essays in Where's the Evidence? focus on problems ignored. This book is a unique collection of critical andw controversial essays on intractable ethical issues and evidence-based problems in modern medicine. Most, but notr therapeutic disasters. Although it is impossible to prevent all missteps in medicine, the author argues, a hedging strategy using concurrent controls when new therapies are introduced always reduces the number of patients killed or injured. It is dangerous to use treatments widely, he warns, before they are subject to rigorous comparative trials. Additionally, the author points out, questions have emerged about how to wield medicine's new capabilities wisely. How do we draw the line,' he asks 'between "knowing" (the acquisition of new medical information) and "doing" (the application of that new knowledge). What are the long- term consequences (moral, social, economic, and biological) of responding to a demand that medicine always do everything that can be done?' This book now issued in paperback is a collection of critical and controversial essays discussing intractable ethical issues and evidence-based problems in modern medicine. The essays together with responses were published over a ten-year period in the journal Paediatric and Perinatal Medicine. Most of the examples, but not all are taken from perinatal medicine, the field in which the author has worked for many years. The essays are thought provoking and will be of great interest to those involved in the ongoing evidence-based medicine debate. (See selected reviews)

This book presents the proceedings of the KES International Conferences on Innovation in Medicine and Healthcare (KES-InMed-19), held in Split, Croatia, on June 17-19, 2020. Covering a number of key areas, including digital IT architecture in healthcare; advanced ICT for medicine and healthcare; biomedical engineering, trends, research and technologies; and healthcare support systems, this book is a valuable resource for researchers, managers, industrialists and anyone wishing to gain an overview of the latest research in these fields.