Fish, and particularly zebrafish, have become the fastest-growing segment of the research population. They offer several advantages, in terms of biology and technologies to apply, and thus are employed in numerous research fields. Laboratory Fish in Biomedical Research: Biology, Husbandry and Research Applications for Zebrafish, Medaka, Killifish, Swordtail Fish, Cavefish, Stickleback, Goldfish and Danionella Translucida addresses the relevant and increasing need to collect cutting-edge knowledge on husbandry, maintenance, welfare and experimental protocols of the most common freshwater species under standard laboratory conditions.

This book comprehensively reviews the anatomy, physiology, genetics and pathology of laboratory animals as well as the principles and practices of using laboratory animals for biomedical research.It covers the design of buildings used for laboratory animals, quality control of laboratory animals, and toxicology, and discusses various animal models used for human diseases. It also highlights aspects, such as handling and restraint and administration of drugs, as well as breeding and feeding of laboratory animals, and provides guidelines for developing meaningful experiments using laboratory animals.Further, the book discusses various alternatives to animal experiments for drug and chemical testing, including their advantages over the current approaches. Lastly, it examines the potential effect of harmful pathogens on the physiology of laboratory animals and discusses the state of art in in vivo imaging techniques.The book is a useful resource for research scientists, laboratory animal veterinarians, and students of laboratory animal medicine.

Fungal growths affect both human and animal well-being. Many natural pathogens of laboratory animals alter host physiology, rendering the host unsuitable for experimental uses. While the number and prevalence of few pathogens have declined considerably, many still turn up in laboratory animals and represent unwanted variables in research. Investigators using laboratory animals in biomedical experimentation should be aware of the profound effects that many of these agents can have on research. What does the future hold regarding the natural pathogens of laboratory animals? The selection of an animal model must carefully address issues of the type of human disease to mimic, the parameters to follow, and the collection of the appropriate data to answer those questions being asked. Overall, animal models of fungal infection will continue to deepen our understanding of how these infections occur. This book provides a valuable source of information to biological and biomedical scientists and to clinical and doctoral researchers working in the area of fungal infections and diseases of laboratory animal species.

"Laboratory Animal Welfare" provides a comprehensive, up-to-date look into the new science of animal welfare within laboratory research. Animals specifically considered include rodents, cats and dogs, nonhuman primates, agricultural animals, avian animals and aquatic animals.

The book examines the impact of experiment design and environment on animal welfare, as well as emergency situations and euthanasia practices. Readers will benefit from a review of regulations and policy guidelines concerning lab animal use, as well as information on assessing animal welfare. With discussions of the history and ethics of animals in research, and a debate on contemporary and international issues, this book is a go-to resource for laboratory animal welfare.

"Animal Models for the Study of Human Disease" identifies important animal models and assesses the advantages and disadvantages of each model for the study of human disease. Thefirst section addresses how to locate resources, animal alternatives, animal ethics and related issues, much needed information for researchers across the biological sciences and biomedicine.The next sections of the work offers models for disease-oriented topics, including cardiac and pulmonary diseases, aging, infectious diseases, obesity, diabetes, neurological diseases, joint diseases, visual disorders, cancer, hypertension, genetic diseases, and diseases of abuse.
Organized by disease orientation for ease of searchabilityProvides information on locating resources, animal alternatives and animal ethicsCovers a broad range of animal models used in research for human disease"

This vibrant collection delivers a laboratory roadmap of testing cognition in the rodent. While rodents and mazes are the main center and focus of this book, many aspects in the field of learning and memory are discussed and detailed, spanning from the molecular to the human, with every chapter delivering a comprehensive review of historical milestones in order to provide context for past discoveries, new findings, and future studies. Didactic foundations, operational definitions, and theory, as well as practical experimental and apparatus set-up, data analysis, and interpretation instructions are included in the first part of the book, while part two contains step-by-step protocols, troubleshooting, and tips from experts in the field. Authoritative and inspirational, The Maze Book: Theories, Practice, and Protocols for Testing Rodent Cognition serves as a detailed and practical manual for scientists wishing to implement these tools in their laboratories and for scholars interested in this powerful field.

A volume in the Handbook of Experimental Animals series, The Laboratory Primate details the past and present use of primates in biomedical research, and the husbandry, nutritional requirements, behaviour, and breeding of each of the commonly used species. Practical information on regulatory requirements, not available in other texts, is covered. Sections on experimental models cover the major areas of biomedical research, including AIDS, cancer, neurobiology and gene therapy. Assisted reproductive technology, tissue typing, and minimum group sizes for infectious disease/vaccine studies are also included.
* Two-color, user-friendly format, with copious illustrations and color plates
* Includes detailed, well-illustrated sections on gross & microscopic anatomy, common diseases, and special procedures, including surgical techniques

Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.

Handbook of Animal Models of Infection is a complete revision of a three-volume text that was published in 1986. It incorporates the major advances in the field during the past decade, in particular those concerning molecular biological procedures and new models that have been developed. It focuses on both methods and techniques, which makes it an essential and comprehensive reference as well as a benchtop manual. The Handbook will help investigators save time and effort in formulating an approach to test a new potential therapeutic agent or combination of agents for "in vivo" efficacy and to position the therapy for specific infections where it may have therapeutic promise. The book is divided into five sections; the first covering the general methodologies, followed by sections describing experimental bacterial, mycotic, parasitic, and viral infections.
Key Features
* Discusses ethical and safety aspects in an introductory background section
* Covers principles of animal care and current techniques appropriate for the use of animal models of infection
* Details a wide range of animals including rodents, rabbits, cats, and primates
* Provides hands-on descriptions of how to set up the model
* Discusses the major advantages and limitations of each model
* Ensures full coverage of bacterial, fungal, viral, and parasitic infections

Anesthesia and Analgesia in Laboratory Animals focuses on the special anesthetic, analgesic, and postoperative care requirements associated with experimental interventions. Fully revised and updated this new edition provides the reader with agents, methods, and techniques for anesthesia and analgesia that ensure humane, reproducible, and successful procedural outcomes. The content is structured in six sections. The first deals with ethical, regulatory, and scientific considerations. Chapters in this section include US and international regulatory considerations, and optimization of anesthesia and analgesia. Part two is dedicated to the principles of anesthesia and analgesia with chapters covering topics in a non-species-specific way, but with a slant towards laboratory animals, including relevant pharmacology of the agents. Part three covers Anesthetic equipment and monitoring. Section four deals with periprocedural care including dedicated chapters to the assessment and management of pain in laboratory species. Part five provides practical considerations by species, including relevant anatomy, physiology, and behavior of a broad range of lab animal species. Part six closes the book with special topics covering management of chronic pain, fetus and neonate interventions, considerations for in-vivo imaging and the study of pain. Anesthesia and Analgesia in Laboratory Animals is the complete reference for veterinarians involved in lab animal research as well as senior graduate, graduate students, post-docs, and researchers who utilize animals in biomedical research.

This book deals with pathophysiology and pharmacology of spontaneously hypertensive rats (SHR) and describes new trends in SHR research from hemodynamic characteristics to immunological views. It is devoted to the use of tissue culture studies to elaborate SHR characteristics.

Pituitary Disease brings together an international panel of experts who summarize the most recent and exciting advances in the diagnosis and treatment of pituitary disease. Coverage includes pathogenesis and pathology of pituitary tumors; clinical scenarios underpinning each type of pituitary tumor as well as their diagnosis and treatment. It is a useful resource for clinicians, students or researchers with an interest in the pituitary and its disorders.

Maintaining its user-friendly approach, The Care and Feeding of an IACUC: The Organization and Management of an Institutional Animal Care and Use Committee, Second Edition is a handy guide for members of the laboratory animal community looking for a concise, descriptive introduction to what an IACUC is all about and how it operates. The book covers training programs and discusses professional certifications for IACUC administrative and animal care staff. It provides pointers to principal investigators, discusses interactions between IACUCs and other compliance panels, and addresses occupational health and safety programs and the role they play in the overall animal care and use program. This new edition of a bestseller contains new information on international regulations regarding animal subject research and the requirements for an ethics panel review. It discusses outside collaborations, interinstitutional memorandums of understanding, and the differences in regulations between countries. The book also includes a new chapter exploring semiannual program reviews, semiannual inspections, and postapproval monitoring. The contributors provide updated information on the protocol submission process, electronic protocol management systems, and records management-including the essential elements of the protocol form and how to complete it. The book also delves deeply into bioethics. It discusses how IACUCs can ensure that each proposal to use animals in research includes an ethical review with a harm-benefit analysis weighing the expected advancements in human and animal health against the potential harm inflicted to the animal. Also included is a chapter on how to survive a regulatory inspection or an accreditation site visit. All interpretations of the regulations have been reviewed by staff at the NIH Office of Laboratory Animal Welfare (OLAW), the Animal Welfare Information Center (AWIC), and USDA APHIS/Animal Care (AC) for consistency and compliance with the PHS Policy and the USDA Animal Welfare Act Regulations (AWAR).

The third edition of The Laboratory Rat features updated information on a variety of topics, including rats as research models for basic and translational research in areas such as genomics, alcoholism, diabetes, metabolic syndrome, obesity, neuroscience, spinal cord injury, traumatic brain injury, regenerative medicine, and infectious disease. New information related to the husbandry and veterinary care of rats is provided including topics related to nutrition, reproduction, anesthesia and surgery, infectious and noninfectious disease, and the care of surgical and other fragile models. It is a premier source of information on the laboratory rat, this book will be of interest to veterinary and medical students, senior graduate students, postdocs and researchers who utilize animals in biomedical research.

Learn the veterinary technician's role in the care of exotic pets and animals used in biomedical research! Laboratory Animal and Exotic Pet Medicine: Principles and Procedures, 3rd Edition helps you gain the knowledge and skills needed to ensure animal health and well-being. It covers animal husbandry, restraint and handling, and diseases, and provides guidelines to key clinical procedures such as blood collection, medication administration, anesthesia, and diagnostic imaging. Research-related information addresses the ethical concerns of exotic pet ownership, as well as the benefits and humane use of animals in research. Written by noted veterinary technology educator Margi Sirois, this text is a must-have resource for all caretakers of lab animals. Comprehensive coverage prepares you to work with all types of animals by addressing a wide variety of species including rats, mice, rabbits, guinea pigs, ferrets, hamsters, gerbils, nonhuman primates, amphibians, fish, reptiles, birds, farm animals, and cats and dogs; it also covers topics such as animal species, the laboratory setting, regulatory guidelines, and ethical considerations. Consistent organization of each species chapter makes it easy to quickly identify similarities and differences among various laboratory animals. Current information on legal, moral, and ethical issues includes legal requirements, the protocols guiding lab animal use, animal exploitation, and animal rights. Discussion of specific uses for each species in biomedical research provides a perspective that helps you explain the benefits of animal use in providing high-quality research data. Technician Notes highlight important points and provide helpful tips to improve your knowledge and skills. Learning objectives, key points, and chapter review questions make studying easier. NEW! Comprehensive coverage of poultry includes the increasingly popular backyard chickens, as well as commonly performed procedures and in-depth information on housing, restraint, nutrition, common diseases, diagnostics, and therapeutics. NEW photographs show the latest technology available in laboratory and exotic animal medicine.

Animal breeding has been complicated by persisting factors across species, cultures, geography, and time. In Made to Order, Margaret E. Derry explains these factors and other breeding concerns in relation to both animals and society in North America and Europe over the past three centuries. Made to Order addresses how breeding methodology evolved, what characterized the aims of breeding, and the way structures were put in place to regulate the occupation. Illustrated by case studies on important farm animals and companion species, the book presents a synthetic overview of livestock breeding as a whole. It gives considerable emphasis to genetics and animal breeding in the post-1960 period, the relationship between environmental and improvement breeding, and regulation of breeding as seen through pedigrees. In doing so, Made to Order shows how studying the ancient human practice of animal breeding can illuminate the ways in which human thinking, theorizing, and evolving characterize our interactions with all-natural processes.

Electrocardiography of Laboratory Animals, Second Edition, is the only publication covering electrocardiography of laboratory animals. With countries instituting requirements for the care of laboratory animals in research, this publication offers a standard on performing and analyzing ECGs. Topics covered include safety electrocardiography, toxicology, safety pharmacology, and telemetry, all important areas of discussion for biological and medical researchers, veterinarians, zoologists, and students who need to understand the electrocardiography of five species of animals used in research: canines, nonhuman primates, mini pigs, rodents (rats and mice), rabbits and cats.

Laboratory Animal Medicine, Third Edition, is a fully revised publication from the American College of Laboratory Medicine's acclaimed blue book series. It presents an up-to-date volume that offers the most thorough coverage of the biology, health, and care of laboratory animals. The book is organized by species, with new inclusions of chinchillas, birds, and program and employee management, and is written and edited by known experts in the fields. Users will find gold-standard guidance on the study of laboratory animal science, as well as valuable information that applies across all of the biological and biomedical sciences that work with animals.

Praise for the Previous Editions "The author brings in management wisdom from the world outside laboratory animal medicine and veterinary medicine. As a result, there is a rich mixture of the experience of a seasoned professional and the theoretical framework used by schools of management .... I recommend this book to managers and laboratory animal specialists at any stage of their careers." -Franklin M. Loew, DVM, PhD, DACLAM, JAVMA, Vol. 222, No. 6, 2003 "... This book is a good informational resource for any new manager to the field of laboratory management. The information is presented in a way that will keep your interest and stimulate you to think how it can benefit you and the facility in which you work." -Susan K. Cutter, BS, RVT, RLATG, Purdue University, West Lafayette, Indiana, USA, LAMA Review Written in Jerry Silverman's trademark style, Managing the Laboratory Animal Facility, Third Edition provides the reader with sound management theory and associated management practices that are easy to read, easy to understand, easy to implement, and pertinent to the daily management and leadership of laboratory animal facilities. Maintaining the practical focus of previous editions, this greatly expanded volume presents the critical knowledge needed to help you make efficient and effective use of the key resources that are used every day by vivarium managers - people, time, money, and information. New to the Third Edition Incorporating the latest developments in management theory and application, the edition contains approximately 100 pages of new and expanded material. This more detailed coverage: Discusses lean management concepts and practices and their application to laboratory animal science Adds information on many essential topics, especially in human resources management in its treatment of negotiations, influence, and performance reviews Provides a large number of revisions and updates to Appendix 2 in its presentation of Per diem calculations Includes an extensive list of references for further study of specialized topics

Since the publication of the first edition, interest in the field has continued to rise, most notably in pain management. Anesthesia and Analgesia in Laboratory Animals focuses on the special anesthetic, analgesic, and post-operative care requirements associated with experimental surgery. Fully revised and updated this new edition provides the reader with agents, methods, and techniques for anesthesia and analgesia that ensure humane and successful procedural outcomes.
* New to this edition - Section V which deals with new issues covering pain research, ethical issues, legal issues, and imaging studies. S
* New to this edition - Section IV now includes chapters on ferrets, birds, reptiles, amphibians, fish, and invertebrates.
*

The ethical treatment of animals has become an issue of serious moral concern. Many people are challenging long-held assumptions about animals and raising questions about their status and treatment. What is the relationship between humans and animals? Do animals have moral standing? Do we have direct or indirect duties to animals? Does human benefit always outweigh animal suffering? The use of animals for experimentation raises all of these questions in a particularly insistent way. Donna Yarri gives an overview of the current state of the discussion, and presents an argument for significantly restricted animal experimentation. Pointing to the similarities between humans and animals, she argues that the actual differences are differences of degree rather than kind. Animal cognition and animal sentiency together are the basis for the claim that experimental animals do have rights. Examining arguments in the disciplines of ethology, philosophy, science, and theology, Yarri makes a case for placing substantial restrictions on animal experimentation. Grounding her examination in Christian theology, she formulates a more humane approach to animal experimentation. She concludes with a concrete burden-benefit analysis that can serve as the foundation for informed decision-making. The Ethics of Animal Experimentation serves as both a handbook of animal rights theory and a practical guide to navigating the complexities of animal experimentation. As animal experimentation features in an increasing number of scientific endeavors, it is an ethical issue that requires our immediate attention. Yarri's unique contribution forges a path toward an ethical practice of animal experimentation.

Written by the international community's leading experts, Trace Elements in Laboratory Rodents describes the best and most current methods to provide deficient or supplemental trace elements to laboratory animals, as well as how to assay them. The experts warn of the common pitfalls and hidden problems in nutritional testing and how to avoid them. This how-to approach focuses on the technical details that make good, reliable studies. Common as well as rare or recently recognized minerals are described relating to both dietary supplementation and measurement in tissues.
If you are a researcher, professor, or student working in nutrition, food science, biochemistry, or veterinary medicine, you can't afford to be without this excellent hands-on methods manual!

Animal Models for the Study of Human Disease, Second Edition, provides needed information on model sharing, animal alternatives, animal ethics and access to databanks of models, bringing together common descriptions of models for busy researchers across biomedical and biological sciences. Offering easily searchable advantages and disadvantages for each animal model and organized by disease topics, this resource aids researchers in finding the best animal model for research in human disease.

Praise for the Previous Editions "The author brings in management wisdom from the world outside laboratory animal medicine and veterinary medicine. As a result, there is a rich mixture of the experience of a seasoned professional and the theoretical framework used by schools of management .... I recommend this book to managers and laboratory animal specialists at any stage of their careers." -Franklin M. Loew, DVM, PhD, DACLAM, JAVMA, Vol. 222, No. 6, 2003 "... This book is a good informational resource for any new manager to the field of laboratory management. The information is presented in a way that will keep your interest and stimulate you to think how it can benefit you and the facility in which you work." -Susan K. Cutter, BS, RVT, RLATG, Purdue University, West Lafayette, Indiana, USA, LAMA Review Written in Jerry Silverman's trademark style, Managing the Laboratory Animal Facility, Third Edition provides the reader with sound management theory and associated management practices that are easy to read, easy to understand, easy to implement, and pertinent to the daily management and leadership of laboratory animal facilities. Maintaining the practical focus of previous editions, this greatly expanded volume presents the critical knowledge needed to help you make efficient and effective use of the key resources that are used every day by vivarium managers - people, time, money, and information. New to the Third Edition Incorporating the latest developments in management theory and application, the edition contains approximately 100 pages of new and expanded material. This more detailed coverage: Discusses lean management concepts and practices and their application to laboratory animal science Adds information on many essential topics, especially in human resources management in its treatment of negotiations, influence, and performance reviews Provides a large number of revisions and updates to Appendix 2 in its presentation of Per diem calculations Includes an extensive list of references for further study of specialized topics

Modeling Neuropsychiatric Disorders in Laboratory Animals serves as a guide for students and basic investigators in the fields of behavioral sciences, psychology, neuroscience, psychiatry, and other professionals interested in the use of animal models in preclinical research related to human neuropsychiatric disorders. The text focuses on the rationale and theory of using animal behavior, both pathological and normal, as a tool for understanding the neural underpinnings of neuropsychiatric disorders. Chapters contain discussions on both classical and modern views on the validation of animal models for neuropsychiatric disorders, also discussing the utility of endophenotypes in modeling neuropsychiatric disease. Subsequent chapters deal with four specific classes of disorders, including anxiety disorders, depressive disorders, obsessive-compulsive and related disorders. Final sections discuss the future for the development, validation, and use of animal models in basic and preclinical research.