Suturing Principles and Techniques in Laboratory Animal Surgery: Manual introduces the basic principles of, and the materials and instruments required for, suturing and wound closure on animals in a research or veterinary facility. The manual provides descriptions and pictures portraying proper tissue handling and aseptic technique during wound closure, the correct hand placement and use of instruments, and the needle placement on the needle holder. The manual also provides in-depth coverage of various suture patterns and associated principles for proper knot tying, including step-by-step descriptions and handouts for each suture pattern. The downloadable resources provide descriptive narrative and step-by-step video demonstrations for each of the suturing techniques while portraying proper tissue handling and aseptic technique. Each suture pattern description includes the common name, principal use, and detailed instructions on how to complete the pattern. An audible narration accompanies the video clip for each suture pattern on the downloadable resources, with a written copy of the narration provided in the book along with each pattern description. The suture pattern handout includes the common name, principal use, brief pattern description, and sequential instructions for each pattern, accompanied by high-quality pictures. This manual and the accompanying downloadable resources help answer suture-related questions and aid readers in making educated decisions about basic wound closure.

Where there are no alternatives to the use of experimental animals in biomedical research, their welfare has to be ensured as far as possible. The aim of this book is to describe the current knowledge about the welfare of laboratory animals. There is previous literature dealing with the welfare of animals in general, as well as literature dealing with the maintenance and use of laboratory animals. This book brings together these two topics, focusing to the general and species-specific needs of laboratory animals in the light of their welfare. The book will provide material for researchers, lecturers, students and technical staff working with laboratory animals. The authors of this book are leading European scientists in laboratory animal science.

The book has two main parts: part one focuses on the general principles of laboratory animal maintenance and experimental use, as well as factors which have to be taken into account when good research is done with animals. The second part is species specific, concentrating on the species most used as laboratory animals. This part gives a comprehensive description of the welfare questions considered to be important for each species under laboratory conditions.

Laboratory animals, including ferrets, play an important role in biomedical research and advances. The humane care and management of these animals remains an ongoing concern. Published in color to provide greater clarity to the techniques and concepts discussed, The Laboratory Ferret presents basic information and common procedures in detail to provide a quick reference for investigators, technicians, and caretakers in the laboratory setting. The book presents a greater understanding of the use of the ferret in research, such as in studies on influenza. It is a valuable, handy reference for experienced individuals and for those without extensive training in working with ferrets.

Praise for the First Edition "This book is easy to read and has many informative illustrations...I would strongly recommend this book to those interested in a broad introduction to the care and use of laboratory guinea pigs as well as to laboratory animal technicians, managers, veterinarians and scientists who do not have direct access to large, well organized animal resource units with extensive library facilities." -John D. Young, Contemporary Topics The Laboratory Guinea Pig provides a compact and well- illustrated introduction to research on the guinea pig. The second edition of this popular handbook facilitates quick reference to the behavioral and physical traits of the guinea pig, as well as practical information on its unique anatomical and biological features. Numerous tables and color illustrations help the reader identify features of anatomy and understand health issues and care requirements. Supplying a stepwise introduction to common laboratory techniques, the new edition of this trusted reference provides guidance that supports the humane care and use of guinea pigs in the laboratory setting.

This bestseller has been an essential book for all those working with laboratory animals since it was first published in 1994. This fourth edition retains all the classic features that have made it a must-have reference including emphasis on best practice in order to improve animal welfare. The contents have been thoroughly updated and reorganised to make sure it is a really practical book for day-to-day use in the laboratory. The first section of the book covers principles applicable to all species, for example husbandry, handling and the education and training required by scientists and technical staff working with animals in the laboratory. Later chapters focus on specific species or groups of species.

New to this edition: - Reflects changes in European legislation and their impact on national legislation- Covers recommendations for the education and training of those carrying out animal experiments across Europe - New chapters on ethical considerations and balancing animal welfare with science- New information on environmental enrichment for laboratory animals- Covers advancements in anaesthesia and analgesia and techniques- Spiral bound for ease-of-use as a bench-top reference

This book is ideal for all personnel carrying out scientific procedures using animals, particularly during training and also for the new researcher. It will also be essential reading for study directors designing research programmes, animal technicians and veterinarians working with laboratory animal species.

Since the popular first edition was published more than a decade ago, the number of swine used in toxicity studies has increased as an alternative to commonly used non-rodent species, such as dogs and primates. A volume in the Laboratory Animal Pocket Reference Series, The Laboratory Swine, Second Edition maintains the high standard set by the previous edition and is poised to continue its legacy as the premier laboratory reference on the care and use of swine in the laboratory.

Emphasizes Humane Care and Use of Laboratory Swine

Addressing the biology, husbandry, management, veterinary care and research applications of both large and miniature swine, this color reference is a complete source of information on the species. It is thoroughly updated and includes a major revision to the anesthetics section. It also places a heightened focus on animal welfare and addresses important considerations related to genetically modified swine. The book is divided into six parts:

• Important Biological Features examines everything from different breeds and behavior, to anatomical and physiological features, as well as digestive, cardiovascular, and pulmonary systems
• Husbandry addresses housing, nutrition, sanitation, transportation, and more
• Management and Quality Assurance looks at the impact of infections on animal research using swine, zoonotic diseases, legal regulations, genetic monitoring, and more
• Veterinary Care covers a variety of topics, such as clinical examination, diseases, pain recognition, and post-operative management
• Experimental Techniques addresses restraint, sampling techniques, basic surgical procedures, and other issues
• Resources provides extensive references for further study, including handbooks, journals, and websites

The Laboratory Swine, Second Edition is ideal for animal caretakers, technicians, investigators, and laboratory animal veterinarians as a single-source reference that contributes to the humane care and use of swine in research.

This book comprehensively reviews the anatomy, physiology, genetics and pathology of laboratory animals as well as the principles and practices of using laboratory animals for biomedical research.It covers the design of buildings used for laboratory animals, quality control of laboratory animals, and toxicology, and discusses various animal models used for human diseases. It also highlights aspects, such as handling and restraint and administration of drugs, as well as breeding and feeding of laboratory animals, and provides guidelines for developing meaningful experiments using laboratory animals.Further, the book discusses various alternatives to animal experiments for drug and chemical testing, including their advantages over the current approaches. Lastly, it examines the potential effect of harmful pathogens on the physiology of laboratory animals and discusses the state of art in in vivo imaging techniques.The book is a useful resource for research scientists, laboratory animal veterinarians, and students of laboratory animal medicine.

Contemporary, thought-provoking yet utterly practical, this book provides an introductory text covering the use and misuse of behaviour tests applied to animals. By including illustrative examples from a variety of species, the book inspires the animal scientist to think about what a given behavioural test can be used for and how the results can be interpreted. This text includes: the dos and don'ts of running behaviour tests and interpreting the results; many clear, simple illustrations which make the information readily accessible, down to earth, practical advice yet a thorough, evidence-based approach; information on behaviour tests for a whole range of species from companion, farm, zoo, laboratory and wild animals; succinct yet comprehensive text, designed to be read cover to cover and stimulate further reading. This book is an essential item in the researcher's toolkit when embarking on and devising any animal behaviour test and is valuable to students, established researchers, teachers and practitioners of applied ethology, animal welfare science, and veterinary science.

Ever since its establishment by USDA regulation in the mid-1980s, the Institutional Animal Care and Use Committee (IACUC) has evolved as the premier instrument of animal welfare oversight within research institutions in the United States. As biomedical research continuously grows, the role and impact of the IACUC has increased in scope and complexity. The IACUC Handbook has become "the Bible" for individuals when the time comes for them to serve on their institution's IACUC. It provides a foundation for understanding and implementing the many and varied responsibilities of this committee. This Third Edition comprehensively addresses the significant changes in the pertinent regulatory environment and interpretation of applicable federal laws, regulations, and policies. It provides multiple references and commentary on the new edition of the Guide for the Care and Use of Laboratory Animals, the new AVMA Guidelines for the Euthanasia of Animals: 2013 Edition, and the Office of Laboratory Animal Welfare's Frequently Asked Questions. The Third Edition also features an updated survey of IACUC practices from institutions around the United States, offering wisdom gained from their experience. In addition, it includes a chapter that provides an international perspective on how animal welfare reviews can function in other countries.

This guide was created especially for individuals performing research with cats whose duties include animal facility management, animal husbandry, regulatory compliance, and technical procedures involved with their research. Basic information and common procedures are presented in detail.

"The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents" is a single volume, comprehensive book sanctioned by the American College of Laboratory Animal Medicine (ACLAM), covering the rabbit, guinea pig, hamster, gerbil and other rodents often used in research. This well-illustrated reference won a 2012 PROSE Award for Best Single Volume Reference in Science from the Association of American Publishers. The book includes basic biology, anatomy, physiology, behavior, infectious and noninfectious diseases, husbandry and breeding, common experimental methods, and use of the species as a research model. With many expert contributors, this will be an extremely valuable publication for biomedical researchers, laboratory animal veterinarians and other professionals engaged in laboratory animal science.
2012 PROSE Award winner for Best Single Volume Reference in Science from the Association of American PublishersOne-stop resource for advancements in the humane and responsible care of: rabbit, guinea pig, hamster, gerbil, chinchilla, deer mouse, kangaroo rat, cotton rat, sand rat, and deguIncludes up-to-date, common experimental methodsOrganized by species for easy access during bench research

Everyone has a view about animal ethics. Each of us, for example, has an opinion about whether we should eat meat; whether animals should be used for scientific research, or whether the use of animals in sport is acceptable. But very few of us stop to wonder about the basis of our views, or to rationalise them. In this book, Madeleine Campbell aims to enable us to do so, by addressing a series of questions such as `When does animal use become abuse?'; `Why do we treat some animals differently from others?'; `Are there some things which we should never do to animals?', and `Just because we can, should we?'. Drawing on her experience as a Veterinarian; a European Diplomate in Animal Welfare Science, Ethics and Law; a researcher and teacher, the author takes ethical argument beyond academia and applies it to the question which currently dominates societal debate about human-animal interactions: what (if anything) is a reasonable use of an animal? Animals, Ethics, and Us offers a stripped back, balanced and moderate perspective, based on logical argument, philosophical principles and sound science. It is a thought-provoking read aimed at a broad readership including informed owners and animal enthusiasts, as well as useful a primer for students of animal ethics, welfare and veterinary medicine.

A respected resource for decades, the Guide for the Care and Use of Laboratory Animals has been updated by a committee of experts, taking into consideration input from the scientific and laboratory animal communities and the public at large. The Guide incorporates new scientific information on common laboratory animals, including aquatic species, and includes extensive references. It is organized around major components of animal use: *Key concepts of animal care and use. The Guide sets the framework for the humane care and use of laboratory animals. *Animal care and use program. The Guide discusses the concept of a broad Program of Animal Care and Use, including roles and responsibilities of the Institutional Official, Attending Veterinarian and the Institutional Animal Care and Use Committee. *Animal environment, husbandry, and management. A chapter on this topic is now divided into sections on terrestrial and aquatic animals and provides recommendations for housing and environment, husbandry, behavioral and population management, and more. *Veterinary care. The Guide discusses veterinary care and the responsibilities of the Attending Veterinarian. It includes recommendations on animal procurement and transportation, preventive medicine (including animal biosecurity), and clinical care and management. The Guide addresses distress and pain recognition and relief, and issues surrounding euthanasia. *Physical plant. The Guide identifies design issues, providing construction guidelines for functional areas; considerations such as drainage, vibration and noise control, and environmental monitoring; and specialized facilities for animal housing and research needs. The Guide for the Care and Use of Laboratory Animals provides a framework for the judgments required in the management of animal facilities. This updated and expanded resource of proven value will be important to scientists and researchers, veterinarians, animal care personnel, facilities managers, institutional administrators, policy makers involved in research issues, and animal welfare advocates.

For critical care of laboratory rodents, there is a scarcity of sources for comprehensive, feasible, and response-oriented information on clinical interventions specific to spontaneous and induced models of disease. With the more complex cases that need critical care management, many treatment approaches to veterinary emergencies cannot be applied directly to the laboratory rodent. The first text of its kind devoted to the challenges of critical care management for laboratory rodents, Critical Care Management for Laboratory Mice and Rats provides a specialized resource for all veterinary, husbandry, technical, and research professionals who utilize rodent models for biomedical research. The book covers the varied approaches to laboratory rodent patient care, health assessments, characteristics of specific disease models, monitoring and scoring of disease parameters, and humane interventions. Giving primary consideration to preservation of animal health and welfare, the text also considers how best to balance welfare with the achievement of proposed scientific objectives. Organized into five chapters, this full-color book covers the following topics: General Approaches for Critical Care Critical Care Management for Laboratory Mice Critical Care Management for Laboratory Rats Special Considerations for Critical Care Management in Laboratory Rodents Resources and Additional Information The author provides treatment guidelines with the expectation that they will be applied with apt professional judgment, allowing for further modification of clinical recommendations for improved patient-based care and welfare for research animals.

Animal welfare is attracting increasing interest worldwide, but particularly from those in developed countries, who now have the knowledge and resources to be able to offer the b est management systems for their farm animals, as well as potentially being able to offer plentiful resources for companion, zoo and laboratory animals. The increased attention given to animal welfare in the West derives la rgely from the fact that the relentless pursuit of financial reward and efficiency has lead to the development of intensive animal production systems that offend the conscience of many consumers in those countries. In devel oping countries, human survival is still a daily uncertainty, so that provision fo r animal welfare has to be balanced against human welfare. Welfare is usua lly provided for only if it supports the output of the animal, be it food, work, clothing, sport or companionship. In reality there are resources for all if they are properly husbanded in both developing and developed countries. The inequitable division of the world s riches creates physical and psychological poverty for humans and animals alike in all sectors of the world. Liv estock are the world s biggest land user (FAO, 2002) and the population, particularly of monogastric animals, is increasing rapidly to meet the need of an expanding human population. Populations of animals managed by humans are therefore increasing worldwide, so there is the tendency to allocate fewer resources to each one."

v Preface The quality of animal models used in biomedical research has increased substantially since the first successful microinjection of foreign DNA frag ments into a mouse zygote in the early 1980s. New well-defined animal models, developed either by micro injection techniques or by homologous recombination in embryonic stem (ES) cells, have become widely available and several classical animal models have now been replaced by these transgenic models. Transgenic animals also fulfill increasingly important roles in other fields, like livestock production or in biopharming. The potential benefits of this technology are overwhelming. It is not surprising that many research groups have rushed to jump on this wagon of scientific progress. However, no matter how important these developments are, they can not relieve us of the moral obligations towards the animals. It is generally recognized that animal use is allowed only if the harmful effects are out weighed by the potential benefits of the experimental results. But what are the harmful effects? What is the impact of trans genesis on the well-being of animals? In order to gain more insight into this aspect of trans genesis the European Commission (DGXII) has funded a 12-month project with the aim of col lecting information on the production and use of transgenic animals in EU Member States and, more specifically, to obtain information as to what extent specific welfare problems may arise and whether or not studies are being performed on the incidence of welfare problems."

1. 1 Experimental Diabetes in the Sand Rat 1. 1. 1 Zoology Experimental diabetes has been used in numerous animals, of which rodents have been predominant because of their ease of maintenance and handling (Herberg 1979), but some species of ape and monkey have also occasionally been used (Brunk 1971; Malaisse and Malaisse-Lagae 1970). Herberg et al. (1976) divided rodent species into two groups based on the similarity of their metabolic response to the induction of diabetes to the onset of diabetes mellitus in man. In one of these groups (mouse, Mongolian gerbil, Tuco tuca), the response of the metabolism to the induction of diabetes was similar to the changes seen in the onset of adult-type diabetes mellitus in man; while the other group, which included the sand rat, spiny mouse, Chinese hamster, and Djungarian hamster, responded to diabetes induction in a way suggestive of juvenile insulin-dependent diabetes. Such a subdivision is not without prob lems, however, particularly since difference occur not only between the various species of a group (Herberg et al. 1976), but, in our experience, also between animals of the same species (for instance the sand rat). Hence, the zoology of animals used for experimental purposes must also be taken into account. 1 The sand rat is classified as follows: Species: obesus Genus: Psammomys Subfamily: Gerbillinae Family: Cricetidae Suborder: Myomorpha Order: Rodentia Subclass: Placentalia Class: Mammalia Subphylum: Vertebrata Phylum: Chordata Psammomys obesus was first described by the zoologist P. J."

This combination manual and downloadable resources provide much-needed training on the proper handling of rats used in biomedical research. The downloadable resources include narrated video clips that demonstrate and describe each procedural technique. The manual contains handouts with color illustrations and descriptive text for each technique, including the purpose and application of the procedure, recommended skills, and necessary supplies. It can be used as a training resource and refresher for lab animal veterinarians, veterinary technicians, animal care staff, trainers, and research investigators and staff who work with rats.

Key features Contains 28 updated tables designed as quick, easy-to-use references for New and Old World species Provides over 100 photographs and illustrations, most now in color, depicting aspects of nonhuman primate biology, behavior, management practices, diseases, and technical procedures Gives a concise overview of regulatory considerations for the use of nonhuman primates in biomedical research Expands the Veterinary Care chapter to include new sections on nutritional support, behavioral conditions, dental care, and updated information on anesthetic and analgesic drugs Presents step-by-step descriptions of common and advanced sampling techniques Includes extensive resource lists for vendors of animals, feed, sanitation supplies, caging, anesthetic equipment, and veterinary and research supplies Extensively updated to include current literature, The Laboratory Nonhuman Primate, Second Edition, continues to serve as a quick reference source for technicians, caretakers, veterinarians, researchers, and students working with primates in biomedical research. It provides details on basic husbandry and covers biologic characteristics, regulatory compliance, common diseases, and anesthetic management. The text gives easy-to-follow descriptions of basic technical procedures including restraint, intubation, tuberculin skin testing, and collection of blood and urine samples. It also reviews advanced sampling procedures including collection of bone marrow, cerebrospinal fluid, bronchoalveolar lavage fluid, and rectal mucosal biopsy. The Laboratory Nonhuman Primate presents information in a clear, concise format to allow readers to incorporate concepts and techniques into the standard operating procedures of a facility.

This guide was created especially for individuals performing research with cats whose duties include animal facility management, animal husbandry, regulatory compliance, and technical procedures involved with their research. Basic information and common procedures are presented in detail.

Key features: Serves as the detailed, authoritative source of the clinical chemistry of the most commonly used laboratory animals Includes detailed chapters dedicated to descriptions of clinical chemistry-related topics specific to each laboratory species as well as organ/class-specific chapters Presents information regarding evaluation and interpretation of a variety of individual clinical chemistry end points Concludes with detailed chapters dedicated to descriptions of statistical analyses and biomarker development of clinical chemistry-related topics Provides extensive reference lists at the end of each chapter to facilitate further study Extensively updated and expanded since the publication of Walter F. Loeb and Fred W. Quimby's second edition in 1999, the new The Clinical Chemistry of Laboratory Animals, Third Edition continues as the most comprehensive reference on in vivo animal studies. By organizing the book into species- and organ/class-specific chapters, this book provides information to enable a conceptual understanding of clinical chemistry across laboratory species as well as information on evaluation and interpretation of clinical chemistry data relevant to specific organ systems. Now sponsored by the American College of Laboratory Animal Medicine (ACLAM), this well-respected resource includes chapters on multiple laboratory species and provides pertinent information on their unique physiological characteristics, methods for sample collection, and preanalytical sources of variation for the particular species. Basic methodology for common procedures for each species is also discussed. New Chapters in the Third Edition Include: The Laboratory Zebrafish and Other Fishes Evaluation of Cardiovascular and Pulmonary Function and Injury Evaluation of Skeletal Muscle Function and Injury Evaluation of Bone Function and Injury Vitamins Development of Biomarkers Statistical Methods The Clinical Chemistry of Laboratory Animals, Third Edition is intended as a reference for use by veterinary students, clinical veterinarians, verterinary toxicologists, veterinary clinical pathologists, and laboratory animal veterinarians to aid in study design, collection of samples, and interpretation of clinical chemistry data for laboratory species.

There are many ongoing debates within and about the subject of animal welfare. This book distils some of the major themes of current debate into one volume, edited by internationally known names in the field of animal welfare. Each chapter is written by one or more leading experts who discuss, in an even-handed way, a provocative topic that will be of interest to anyone concerned with animal welfare. Issues covered include tail docking, farm animal production, neutering of feral cats and the need to conserve habitats of native wild animals in the face of threats from non-native species. Chapters address the different values and priorities involved in dealing with these issues, including scientific and more explicit ethical approaches. Each chapter ends with questions for discussion that may help readers to engage with these dilemmas.

Laboratory animals, including birds, play an important role in biomedical research. The humane care and management of these animals is an ongoing concern. A new addition to the acclaimed Laboratory Animal Pocket Reference series, The Laboratory Bird is the first publication dedicated to the care and use of avian species in the research setting. Covering avian species such as chickens, ducks, doves, parrots, and songbirds that are commonly used as research models, the book is divided into focused chapters that cover a broad range of topics, including: General avian biology and physiology Husbandry Regulations and regulatory compliance regarding the use of birds in research Experimental methods Veterinary care Along with discussing applicable regulations, the book also details issues of health management and quarantine approaches. The final chapter provides resources such as organizations, publications, vendors, and diagnostic laboratories. With its focus on the care of a diverse group of avian species in biomedical research settings, The Laboratory Bird is a valuable reference for animal care and veterinary technicians, laboratory animal veterinarians, trainees in laboratory animal medicine, and research staff members, as well as individuals involved in laboratory work who lack experience in working with birds.

Contemporary, thought-provoking yet utterly practical, this book provides an introductory text covering the use and misuse of behaviour tests applied to animals. By including illustrative examples from a variety of species, the book inspires the animal scientist to think about what a given behavioural test can be used for and how the results can be interpreted. This text includes: the dos and don'ts of running behaviour tests and interpreting the results; many clear, simple illustrations which make the information readily accessible, down to earth, practical advice yet a thorough, evidence-based approach; information on behaviour tests for a whole range of species from companion, farm, zoo, laboratory and wild animals; succinct yet comprehensive text, designed to be read cover to cover and stimulate further reading. This book is an essential item in the researcher's toolkit when embarking on and devising any animal behaviour test and is valuable to students, established researchers, teachers and practitioners of applied ethology, animal welfare science, and veterinary science.

With laboratory animals, especially rabbits, playing such an important role in biomedical research, the humane care of these animals is an ongoing concern. The Laboratory Rabbit, Second Edition presents basic information and common procedures in detail to provide a quick reference for caretakers, technicians, and researchers in a laboratory setting.

Now in full color, the second edition of this book illustrates management practices and technical procedures with numerous figures and tables. It includes updated tables on anesthetic agents, methods of euthanasia, recommended needle sizes, injection sites, approximate values for injection, and sedative and immobilization agents. Plastic comb-bound for convenient and frequent use, this guide also provides sources and suppliers of additional information on rabbits, feed, and sanitation supplies.