The popularity of germ-free animal models, particularly mice, for
investigation of human physiology and disease has recently
exploded. Gnotobiotic Mouse Technology: An Illustrated Guide
provides the first manual for the maintenance, husbandry, and
experimental manipulation of germ-free and gnotobiotic mice. It
includes information on all aspects of establishing and operating a
germ-free mouse research facility, from basic principles and
equipment to detailed instructions for assembling and maintaining
isolators, sterilizing supplies, handling animals, and monitoring
sterility. The book breaks down techniques and procedures into
modules, each of which contains a step-by-step practical and visual
guide to a set of related procedures. Each description consists of
a materials list, introduction, and general overview, followed by a
thoroughly illustrated walkthrough of the steps for each procedure.
The descriptions end with troubleshooting tips. Technical chapters
are heavily illustrated and include notations of potential pitfalls
and alternatives. Other chapters discuss management procedures and
practices such as the hiring and training of personnel, setting fee
schedules, and record keeping. This book is a landmark resource for
establishing and maintaining a facility for germ-free mouse
research. Useful to both technicians and investigators, it presents
every step necessary to establish a successful facility. It also
gives direction in expanding multi-user facilities and applying new
technologies to your current practices.
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