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MT-PCR - A rapid, reliable and effective tool for assessing toxic 'algal' blooms in Victorian water supplies - Aiding protection and preservation (Paperback)
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MT-PCR - A rapid, reliable and effective tool for assessing toxic 'algal' blooms in Victorian water supplies - Aiding protection and preservation (Paperback)
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This project addressed a need for a sensitive, accurate and
reliable testing method to aid assessment of the toxicity of algal
blooms and assist water management. Increasingly, diagnostic
dilemmas are resolved through the use of DNA-based technologies
which often provide high sensitivity and specificity and are
efficient both in terms of costs and time. However to date, no such
test was available to the Victorian water industries. This project
sought to bridge this gap by developing an automated DNA-based
diagnostic assay for cyanobacterial bloom assessment blooms in
Victorian waters. The assay exceeds expectation in its ability to
accurately quantify levels of toxigenic cyanobacteria in bloom
samples, retains exceptionally high specificity and sensitivity and
each assay out-performs common conventional PCR approaches
established in the literature. Four toxigen assays (microcystin,
nodularin, cylindrospermopsin and saxitoxin) were designed, tested
and optimised. This book is co-published with Water Research
Australia Authors: Aaron Jex, Louise Baker and Raechel Littman,
University of Melbourne
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