The objective of the present investigation was to establish in
vitro culture and plant regeneration methods from leaf base and
rhizome bud explants of Z. officinale. The MS medium with 1.0 mg/l
2, 4-D proved to be the best for callus induction from leaf base
explants. Shoot regeneration was achieved after subculturing the
calli in different media formulation and 8.0 mg/l BA with 0.2 mg/l
2, 4-D was found to be the best for multiple shoot regeneration
from callus through organogenesis. MS medium supplemented with 5.0
mg/l NAA was the best formulation for successful culture
establishment as well as shoot proliferation from rhizome bud
explant. Multiple shoot proliferation was noticed at 4th subculture
in medium with 5.0 mg/l NAA and shoot proliferation was increased
with the increased number of subculture. Activated charcoal (AC)
enhanced multiple shoot proliferation and the optimum pH level for
shoot formation was 5.5 - 6.0 in the medium. MS + 4.0 mg/l NAA
proved to be the best for root induction. Rooted shoots (plantlets)
were gradually acclimatized and successfully established in polybag
soil.
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