Retinal Gene Therapy - Methods and Protocols (Hardcover, 1st ed. 2018)

This detailed volume describes a spectrum of methods and protocols that can be used for the bench-to-bedside development and evaluation of retinal gene therapy. Methods for the successful delivery of these gene therapy vector systems to the retina are examined, as well as assays to test the efficacy in vitro in cell cultures, for gene augmentation and gene editing in vivo on rodents, pigs, and monkey retinas, and on human retinal explants as well as in human clinical studies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Retinal Gene Therapy: Methods and Protocols provides a wide range of readers from students to research experts with vital information on ocular gene therapy vector technology, in vitro and in vivo biological assays, and clinical protocols, to promote further studies for the benefit of children and adults with inherited retinal disease.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 1715
Release date:	November 2017
First published:	2018
Editors:	Camiel J.F. Boon • Jan Wijnholds
Dimensions:	254 x 178mm (L x W)
Format:	Hardcover
Pages:	380
Edition:	1st ed. 2018
ISBN-13:	978-1-4939-7521-1
Categories:	Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics Promotions
LSN:	1-4939-7521-8
Barcode:	9781493975211

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