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High-Throughput RNAi Screening - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2016)
Loot Price: R3,729
Discovery Miles 37 290
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High-Throughput RNAi Screening - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2016)
Series: Methods in Molecular Biology, 1470
Expected to ship within 10 - 15 working days
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High-throughput RNAi screening remains one of the most widely used
technologies to perform target identification and validation
studies in an unbiased manner. These assays are equally important
for research and development across academic, biotech, and
pharmaceutical industries. The success of these screening efforts
is dependent on robust methodologies to perform these screens. In
High-Throughput RNAi Screening: Methods and Protocols, expert
researchers in the field share protocols and methods for performing
high-throughput RNAi (HT-RNAi) screens. These include the use of
various RNAi platforms and delivery methods in mammalian and
non-mammalian systems, whole organism and cell models, and various
applications, such as drug sensitizer identification. Finally, the
book examines the latest advancements in the fields of assay
development, library screening, data analysis, and hit selection.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Cutting-edge and
thorough, High-Throughput RNAi Screening: Methods and Protocols
provides a comprehensive source of protocols and other necessary
information to make robust and successful assays possible for all
who wish to apply HT-RNAi in their research.
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