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Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis (Paperback, Softcover reprint of the original 1st ed. 2014)
Loot Price: R3,047
Discovery Miles 30 470
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Applications of Monolithic Column and Isotope Dimethylation Labeling in Shotgun Proteome Analysis (Paperback, Softcover reprint of the original 1st ed. 2014)
Series: Springer Theses
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Total price: R3,067
Discovery Miles: 30 670
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In this thesis, the author outlines the development of new
monolithic columns and isotope dimethyl labeling strategies and
their applications in high-performance proteome analyses. Though
different types of monolithic columns have been widely developed
for chromatography and electrophoresis separation, their
application in proteomics for complex peptide mixtures separation
is still a challenge. The author discusses the preparation of new
monolithic columns and optimization of chromatography separation
capability to improve coverage and accuracy of proteome analysis.
Further, the author describes a novel online multidimensional
chromatography system combined with automated online isotope
labeling, which significantly improves the throughput, sensitivity
and accuracy of quantitative proteomics. In addition to the
development of new technologies, the author investigates the
proteome and phosphoproteome expression changes of clinical
hepatocellular carcinoma tissues and the hippocampi of mice with
Alzheimer’s disease. The work in this thesis has led to several
publications in high-profile journals in the fields of analytical
chemistry and proteome research.
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