This volume in the well-established Methods in Enzymology series
features methods for the study of lipids using mass spectrometry
techniques. Articles in this volume cover topics such as
Phospholipase A1 assays using a radio-labeled substrate and mass
spectrometry; Real-time Cell Assays of Phospholipases A2 Using
Fluorogenic Phospholipids; Analysis and Pharmacological Targeting
of Phospholipase C a interactions with G proteins; Biochemical
Analysis of Phospholipase D.; Measurement of
Autotaxin/Lysophospholipase D Activity; Platelet-Activating Factor;
Quantitative measurement of PtdIns(3,4,5)P3; Measuring
Phosphorylated Akt And Other Phosphoinositide 3-Kinase-Regulated
Phosphoproteins In Primary Lymphocytes; Regulation of
Phosphatidylinositol 4-Phosphate 5-Kinase activity by partner
proteins; Biochemical Analysis of Inositol Phosphate Kinases;
Analysis of the phosphoinositides and their aqueous metabolites;
Combination of C17-sphingoid base homologues and mass spectrometry
analysis as a new approach to study sphingolipid metabolism;
Measurement of mammalian sphingosine-1-phosphate phosphohydrolase
activity in vitro and in vivo; A rapid and sensitive method to
measure secretion of sphingosine-1-phosphate; Ceramide Kinase and
Ceramide-1-Phosphate; Measurement of Mammalian Diacylglycerol
Kinase Activity in vitro and in Cells; Lipid Phosphate Phosphatases
from Saccharomyces cerevisiae.
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