Micropropagation from shoot tips of one-month-old suckers of six
banana cultivars and subsequent field evaluation for commercial
exploitation, were studied. Among the various growth regulators,
the medium with 5 mg/l BAP was found to be the most effective for
micropropagation. Among the six banana cultivars, Sabari (AAB
genome) was the most responsive genotype to micropropagation.
Micropropagated plants of all cultivars showed significantly
superior performances for yield and yield contributing characters
over sucker-derived plants. In the second part induction of callus
from the male flowers and subsequent somatic embryogenesis were
tested. Among the different media formulations, the medium
fortified with 4 mg/l 2,4-D + 1 mg/l each of IAA, NAA and biotin
was found to be the most effective growth regulator formulation for
callus induction and growth. Among the five cultivars male flowers
of Sabari were found to show the highest response to callus
induction and subsequent embryo formation. Somatic embryo derived
plants of all banana cultivars at maturity exhibited wide range of
variation in yield and yield contributing characters.
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