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Plant Organogenesis - Methods and Protocols (Hardcover, 2013 ed.)
Loot Price: R5,072
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Plant Organogenesis - Methods and Protocols (Hardcover, 2013 ed.)
Series: Methods in Molecular Biology, 959
Expected to ship within 12 - 17 working days
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Organogenesis entails the regulation of cell division, cell
expansion, cell and tissue type differentiation, and patterning of
the organ as a whole. It is essential to gain insight into how
organs are initiated and how they develop. However, this very often
is subject to technical difficulties as these processes take place
embedded deep in tissues or are difficult to access or visualize.
To achieve this, we need specialized techniques such as those
concisely illustrated in Plant Organogenesis: Methods and
Protocols. Chapters address topics such as how to study and image
the structure of ovules and embryos of Arabidopsis thaliana, tools
to establish cell lineages in order to visualize the contribution
of each cell and cell division to the building of a mature organ,
approaches to study the totipotency of several plant cells,
techniques such as the use of fluorescence-activated cell sorting
(FACS) to analyse transcriptomes and hormone levels in Arabidopsis,
methods to investigate organogenesis in economically important
crops, and computer-based approaches to bring everything together.
Written in the successful Methods in Molecular Biology (TM) series
format, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step,
readily reproducible protocols, and notes on troubleshooting and
avoiding known pitfalls. Authoritative and easily accessible, Plant
Organogenesis: Methods and Protocols serves both professionals and
novices with its well-honed methodologies in an effort to further
our knowledge of this fascinating research field.
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