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Tissue Culture of Trees (Paperback, 1983 ed.)
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Tissue Culture of Trees (Paperback, 1983 ed.)
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1 John H. Dodds The culture offragmen ts of plant tissue is not a
particularly new science, in fact as long ago as 1893 Rechinger
(1893) described the formation of callus on isolated fragments of
stems and roots. The culture of plant tissues in vitro on a
nutrient medium was performed by Haberlandt (1902), however, his
attempts were unsuccessful because he chose too simple a medium
that lacked critical growth factors. Over the last fifty years
there has been a surge of development in plant tissue culture
techniques and a host of techniques are now avail able (Dodds and
Roberts, 1982). The major areas are as follows. Callus Culture
Callus is a rather ill-dermed material. but is usually described as
an un organised proliferating mass of tissue. Although callus
cultures have a great deal of potential in the biotechnological
aspects of tissue culture, i.e. secondary product formation, they
are not very suitable for plant propagation. The key reason for
their unsuitability is that genetic aber rations occur during
mitotic divisions in callus growth (D'amato.l965). The aberrations
can be of a major type, such as aneuploidy or endo reduplication.
It follows therefore that the genetic status of the re generated
plants is different from that of the parent type. In general terms
this genetic instability is undesirable, but there are occasions
when a callus stage can be purposely included to diversify the
genetic base of the crop.
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