The integration of micro-fluidics, electrical impedance
spectroscopy and stochastic estimation will lead to a device with
enhanced detection capabilities. The goal of this thesis was to
build a micro-fluidic electrical impedance measurement device that
can be used in combination with a stochastic estimator to
accurately identify living cells. A microdevice capable of making
impedance measurements on individual living cells was designed and
built using a series of standard microelectronic fabrication
techniques. A microchannel was patterned in SU-8 photoresist
between two gold microelectrodes on a two inch Pyrex 7740 wafer.
The design process, the fabrication techniques for the
microchannel, the fluid port fabrication and the cover slip bonding
processes are described in detail. Small glass cover slips were
bonded to the wafer using Loctite 3301 adhesive. Impedance
measurements of single cells, in the microchannel device, were made
using a HP4194A impedance analyzer. Preliminary analysis of the
impedance data suggests that Jurkat cells have characteristic
impedance signatures, corresponding to their cell type. The
microdevice that was designed and built for this project should
facilitate future work to implement a stochastic estimation
algorithm capable of single cell identification.
General
| Imprint: |
Biblioscholar
|
| Country of origin: |
United States |
| Release date: |
November 2012 |
| First published: |
November 2012 |
| Authors: |
Karl R. Schwenn
|
| Dimensions: |
246 x 189 x 6mm (L x W x T) |
| Format: |
Paperback - Trade
|
| Pages: |
104 |
| ISBN-13: |
978-1-288-30884-2 |
| Categories: |
Books >
Social sciences >
Education >
General
Promotions
|
| LSN: |
1-288-30884-1 |
| Barcode: |
9781288308842 |
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