This book draws together recent data on both cytoplasmic and
flagellar dyneins and the proteins they interact with, to give
readers a clear picture of what is currently known about the
structure and mechanics of these remarkable macro-molecular
machines. Each chapter is written by active researchers, with a
focus on currently used biophysical, biochemical, and cell
biological methods. This is a useful handbook for frontline
researchers as well as a textbook for advanced students. Besides
comprehensive cover of structural information gained by electron
microscopy, electron cryo-tomography, X-ray crystallography, and
nuclear magnetic resonance, this book provides detailed
descriptions of mechanistic experiments by single-molecule
nanometry. The reports include in vitro studies of the motility of
reconstituted complexes and in vivo studies of organisms expressing
mutant dyneins. The recent technical improvements described, which
have played an important part in recent advances, include the
expression and preparation of recombinant dynein heavy chains or
individual subdomains.
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