The given plant, Solanum tuberosum was grown in the field as well
as in petri plants by seeds.The different explants such as leaf,
node, internodes, bud and roots were taken for micropropagation.
These explants were grown in vitro after thorough sterilization
using different PGR in different media composition.The PGR used
were 2,4-D, BAP and NAA. Their combination were also used i.e.
2,4-D+BAP, BAP+NAA and 2,4-D+NAA in different concentration.The
most effective and suitable media composition was MS (Murashige
& Skoog, 1962) basal medium with BAP (2.0 mg/l) for
regeneration from nodal explant of Solanum tuberosum.The optimum
temperature was found to be 23 02 oC. The micropropagation occurred
more precisely at about 30 (g/l) sucrose concentrations. The most
effective photoperiod for the regeneration of Solanum tuberosum was
16 hours light period. The pH 5.7 was considered to be the best for
the micropropagation of Solanum tuberosum.
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