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Plant Gene Silencing - Methods and Protocols (Hardcover, 2015 ed.)
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Plant Gene Silencing - Methods and Protocols (Hardcover, 2015 ed.)
Series: Methods in Molecular Biology, 1287
Expected to ship within 12 - 17 working days
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This volume provides readers with a comprehensive review of various
gene silencing methodologies and its applications. It provides a
historical overview of gene silencing mechanisms in plants,
vectors, and strategies available for plant gene silencing,
practical applications of gene silencing, bioinformatics tools, and
other resources. In addition to these review chapters, this book
includes methodology for virus-induced gene silencing (VIGS) in
various different plant species, understanding plant stress
responses using VIGS, miRNA identification, DNA interference,
host-induced gene silencing, use of artificial miRNAs for gene
silencing, high throughput RNAi, and more. Written in the highly
successful Methods of Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and key tips on troubleshooting
and avoiding known pitfalls. Thorough and practical, Plant Gene
Silencing: Methods and Protocols seeks to aid scientists to further
understand functional relevance of target genes using gene
silencing methods and use these technologies in commercial plant
varieties.
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