Providing a guide to classical experimental approaches to decipher
splicing mechanisms and experimental strategies that rely on novel
multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing:
Methods and Protocols describes the theory of alternative pre-mRNA
splicing in seven introductory chapters and then introduces
protocols and their theoretical background relevant for a variety
of experimental research. These protocol chapters cover basic
methods to detect splicing events, analyses of alternative pre-mRNA
splicing in vitro and in vivo manipulation of splicing events and
high-throughput and bioinformatic analyses of alternative splicing.
Written in the highly successful Methods in Molecular Biology
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols and tips on
troubleshooting and avoiding known pitfalls. Comprehensive and
practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols
will aid newcomers and seasoned molecular biologists in
understanding the fascinating world of alternative splicing with
the ultimate goal of paving the way for many new discoveries to
come.
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