In the present investigation an attempt was made to develop a large
scale production of planting materials of two Japanese hybrid
cultivars of muskmelon through micropropagation. Seeds of two
popular Japanese muskmelon cultivars were surface sterilized and
cultured on semisolid MS medium supplemented with different
concentrations of GA3. Seed germination was the highest when MS
medium was supplemented with 0.2 mg/l GA3. In the present work, two
explants of two muskmelon cultivars viz. Sweet Gold and Midori
excised from axenic seedlings were cultured for shoot
proliferation. Nodal segments showed better shoot proliferation
than shoot tip explants. The maximum efficiency of shoot
multiplication was recorded in MS medium supplemented with 2.0 mg/l
BA. Maximum number and length of shoots were achieved in case of
nodal explants when cultured on MS medium augmented with 2.0 mg/l
BA. The highest percentage of in vitro grown cultured shoots to
develop roots was obtained when they were cultured in MS rooting
medium containing 0.5 mg/l NAA for two cultivars. Successfully
acclimatized plantlets were transplanted in to field and 80%
plantlets survived.
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