Bacterial Secretion Systems - Methods and Protocols (2nd ed. 2023)

This second edition details new and updated protocols that cover techniques used to study secretion systems. Chapters focus on identifying and localizing the different subunits, defining interactions within subunits, monitoring conformational changes, purifying and imaging of large complexes, defining the assembly pathway by fluorescence microscopy and the role of energy during assembly and/or secretion, identifying secreted effectors as well as using reporters to follow effector transport. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Bacterial Secretion Systems: Methods and Protocols- Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

General

Imprint:	Springer-Verlag New York
Country of origin:	United States
Series:	Methods in Molecular Biology, 2715
Release date:	October 2023
First published:	2023
Editors:	Laure Journet • Eric Cascales
Dimensions:	254 x 178mm (L x W)
Pages:	580
Edition:	2nd ed. 2023
ISBN-13:	978-1-07-163444-8
Categories:	Books
LSN:	1-07-163444-5
Barcode:	9781071634448

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