Manual of Techniques in Invertebrate Pathology, Second Edition,
describes a wide range of techniques used in the identification,
isolation, propagation/cultivation, bioassay, quantification,
preservation, and storage of the major groups of entomopathogens,
including entomophthorales, entomopathogenic fungi,
entomopathogenic bacteria of the Bacilli, Nematode parasites, and
pathogens and parasites of terrestrial molluscs. The book presents
the perspectives of an international group of experts in the fields
of invertebrate pathology, including microbiology, mycology,
virology, nematology, biological control, and integrated pest
management. Organized into 15 chapters, the book covers methods for
the study of virtually every major group of entomopathogen, as well
as methods for discovery and diagnosis of entomopathogens and the
use of complementary methods for microscopy. It discusses the use
of molecular techniques for identifying and determining phylogeny,
factors that contribute to resistance to entomopathogens, and
several other aspects of the science of invertebrate pathology. It
also explains initial handling and diagnosis of diseased
invertebrates, basic techniques in insect virology, and bioassay of
bacterial entomopathogens against insect larvae. In addition, the
reader is introduced to the use of bacteria against soil-inhabiting
insects and preservation of entomopathogenic fungal cultures. The
remaining chapters focus on research methods for entomopathogenic
microsporidia and other protists, how the pathogenicity and
infectivity of entomopathogens to mammals are tested, and
preparations of entomopathogens and diseased specimens for more
detailed study using microscopy. Experienced insect pathologists,
biologists, entomologists, students, biotechnology personnel,
technicians, those working in the biopesticide industry, and
government regulators will find this manual extremely helpful.
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