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Peptidomics - Methods and Protocols (Hardcover, 2010 ed.)
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Peptidomics - Methods and Protocols (Hardcover, 2010 ed.)
Series: Methods in Molecular Biology, 615
Expected to ship within 12 - 17 working days
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Despite being known and studied for years, peptides have never
before attracted enough attention to necessitate the invention of
the term "peptidomics" in order to specify the study of the
complement of peptides from a cell, organelle, tissue or organism.
In Peptidomics: Methods and Protocols, expert researchers present a
comprehensive range of analytical techniques for the analysis of
the peptide contents of complex biological samples with an emphasis
often on higher throughput techniques, suitable for the analysis of
large numbers of peptides typically present in the peptidomes.
Encompassing a number of species ranging from bacteria to man, the
methods presented intensively cover topics such as organism
handling, tissue and organ dissection, cellular and subcellular
fractionation, peptide extraction, fractionation and purification,
structural characterization, molecular cloning, and sequence
analysis. Written in the highly successful Methods in Molecular
Biology (TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Comprehensive and
cutting-edge, Peptidomics: Methods and Protocols brings this ten
year old field fully up-to-date in order to inspire novices and
experts alike with the easy-to-follow practical advice needed to
set up and carry out analysis of the peptide contents of complex
biological samples.
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