Shape your heart to front the hour, but dream not that the hour
will lost. Alfred Tennyson Locksley Hall Sixty YeO!, After Numerous
advances in the life sciences have necessitated the need to develop
gentle, quick and economical methods for separation of
proteins/enzymes. Affmity-based separations, with their high
selectivity, have thus come to be used more frequently. In the
beginning, we just had atrmity chromatography. Today we have
several strategies that employ atrmity interactions for separa-
tion and analysis. This book is an attempt to provide a short
introduction and illustrative protocols for some important and
current affinity-based approaches inthe area ofdownstream
processingofenzymes/proteins. Today, people from a variety of
disciplines have to purify proteins. It is hoped that this book
will provide some quick insight into the various available options
and guide even a novice (in the area ofprotein separation) to
obtain his/her target protein at the level of purity that he/she
desires.
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