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Site-Specific Recombinases - Methods and Protocols (Hardcover, 1st ed. 2017)
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Site-Specific Recombinases - Methods and Protocols (Hardcover, 1st ed. 2017)
Series: Methods in Molecular Biology, 1642
Expected to ship within 12 - 17 working days
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This books aims to provide an up-to-date guide to using
recombinases in the laboratory. Chapters guide the reader through
construction of transgenic animals, recombinase-mediated cassette
exchange (RMCE), spatial control of recombinase expression and in
vivo delivery approaches, immunohistochemistry and
fluorescence-based phenotyping methods, biochemical methods, and
recombinase-based production of minimal DNA vectors. Written in the
highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge,
Site-Specific Recombinases: Methods and Protocols aims to serve as
a valuable resource for working with recombinases, and inspires
further creativity in the field.
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