Jatropha is one of the most important plant species of the world
valued for its biofuel property. The species is primarily
propagated through seeds, and thus the seed yield and oil content
varies significantly. The present work describe the reproducible
and efficient in vitro regeneration method from different types of
explant (Leaf, petiole, cotyledonary leaf and petiole) and source
of explant (in vitro and in vivo) of toxic and non-toxic cultivar
of Jatropha. Besides, providing an efficient, reproducible and
genotype independent regeneration protocol from different types and
source, also provides optimized protocol for successful genetic
transformation of Jatropha leaf explants and molecular
characterization of transformed plants which help in improvement of
this important biofuel plant.
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