A collection of powerful new techniques for oligonucleotide
synthesis and for the use of modified oligonucleotides in
biotechnology. Among the protocol highlights are a novel two-step
process that yields a high purity, less costly, DNA, the synthesis
of phosphorothioates using new sulfur transfer agents, the
synthesis of LNA, peptide conjugation methods to improve cellular
delivery and cell-specific targeting, and triple helix formation.
The applications include using molecular beacons to monitor the PCR
amplification process, nuclease footprinting to study the
sequence-selective binding of small molecules of DNA, nucleic acid
libraries, and the use of small interference RNA (siRNA) as an
inhibitor of gene expression.
General
| Imprint: |
HumanaPress
|
| Country of origin: |
United States |
| Series: |
Methods in Molecular Biology, 288 |
| Release date: |
November 2010 |
| First published: |
2005 |
| Editors: |
Piet Herdewijn
|
| Dimensions: |
229 x 152 x 25mm (L x W x T) |
| Format: |
Paperback
|
| Pages: |
436 |
| Edition: |
Softcover reprint of hardcover 1st ed. 2005 |
| ISBN-13: |
978-1-61737-441-8 |
| Categories: |
Books >
Medicine >
Nursing & ancillary services >
Pharmacy / dispensing
Promotions
|
| LSN: |
1-61737-441-5 |
| Barcode: |
9781617374418 |
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