Metallic starch capped gold nanoparticles have been synthesized by
the reduction of chloroaurate anions AuCl4]- solution with
hydrazine in the aqueous starch and ethylene glycol solution at
room temperature and at atmospheric pressure. The characterization
of synthesized gold nanoparticles by UV-Vis spectroscopy, Scanning
electron microscopy (SEM), X-ray diffraction (XRD) indicate that
average size of pure gold NPs is 5-10 nm, they are spherical in
shape and are pure metallic gold. Starch capped gold nanoparticles
along with tyrosinase enzyme are effectively entrapped within
sol-gel matrix for application in catechol biosensor. Enzymatic
reaction between tyrosinase and catechol results in the formation
of a colored compound which shows characteristic absorbance at 410
nm. Starch@AuNPs based catechol biosensor showed fast response with
linearity for catechol sensing from 1 to 5 mM. Effect of buffer pH
and its concentration was also studied and results showed drastic
changes in absorption intensity with pH and concentration
variation. Along with catechol biosensor, starch capped gold NPs
find their application in glucose, urease and peroxide biosensor,
owing to their stability.
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