Pioneers and leading researchers explain the theory and techniques
of using targeted toxins experimentally. The highly successful use
of the 192 IgG-saporin and ME20.4-saporin immunotoxins to lesion
the cholinergic basal forebrain in order to model the behavior,
anatomy, physiology, and pharmacology of Alzheimer's disease in
animals is treated in detail to give a potential user the knowledge
to comfortably use the techniques involved. The uses of important
new lesioning agents such as anti-DBH-saporin immunotoxin to make
remarkably selective lesions of catecholaminergic neurons,
hypocretin-saporin that can produce narcoleptic animals, and other
saporin conjugates, such as neuropeptide-saporin conjugates for
pain research and cholera toxin B chain-saporin to produce a model
of CNS demyelination are explained by experts in the field.
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