With this valuable practical guide, three members of the Harvard
Stem Cell Institute have compiled and edited the definite handbook
for the exciting new field of human embryonic stem cell research.
The editors have gathered protocols from scientists with extensive
reputation and expertise, describing and comparing currently used
techniques for the culture of human stem cells and discussing the
strengths and weaknesses of the different approaches.
"Human Embryonic Stem Cells: The Practical Handbook" contains
the first centralised collection of methods used in human embryonic
stem cell biology. The book covers the derivation of human stem
cell lines, the obtaining of cells from human stem cell banks, the
culturing and characterisation of the cells, and the
differentiation of the cells "in vitro" and "in vivo." Lastly,
almost all of these protocols can also be used for analyzing and
manipulating induced pluripotency iPS stem cells. This allows an
even greater number of opportunities for those interested in
purusing work in pluripotent stem cells, disease modelling, and
other aspects of basic regenerative medicine research.
The novel and useful focus of this book sets it apart from other
available books: Compares and evaluates the protocols used in
leading laboratories working on human embryonic stem cellsCentred
solely on practical protocols for human (not mouse) embryonic stem
cell researchIncludes extensive troubleshooting sectionsAddresses
the different proclivities and behaviours of individual human
embryonic cell linesContains techniques currently known only to a
small number of specialised laboratories worldwide
This handbook represents an essential source of up-to-date
practical information for all cell and developmental biologists
working with human embryonic stem cells or wishing to enter the
field. It is also essential reading for clinical researchers in
areas such as diabetes, cardiovascular disease, and neurological
diseases.
Praise from the reviews:
.".".a highly readable and useful book... A notable feature of
the book is its air of openness and honesty... This book... will
help many to navigate the uncharted waters of human embryonic stem
cell biology."" BRITISH SOCIETY FOR CELL BIOLOGY
.".". the imaginative solutions in this book can inspire us to
get past our most frustrating limitations."" CELL STEM CELL
.".". the richness in the details of each protocol presented
will certainly encourage more scientists to begin studies of Human
pluripotent stem cells..."" REGENERATIVE MEDICINE
""In this fast-moving field, this handbook] will help drive
advances of more and more researchers." " DIFFERENTIATION
.".".a valuable resource for seasoned and novice researchers...
an excellent addition to the reference collection of any medical
library or research laboratory."" THE AMERICAN MEDICAL
ASSOCIATION
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