The genotyping results of this study suggest that type specific PCR
evaluated in this study may reliably be used in genotyping studies
of HCV. Both type specific PCR and serotyping has advantages and
drawbacks. Serotyping assay have the advantages over molecular
biology based typing methods only in terms of low cost, rapid test
procedure and requirement of a few instruments that would be
present in any clinical laboratory. However, ELISA is less
sensitive and does not allow identification of HCV mixed infection
and subtypes. Furthermore, the serotyping assay cannot
differentiate between current and past HCV infection as the
antibody remains in patient's body for some time (from 2 years to
12 years) after the clearance of the virus. The type-specific PCR
as used in this study is complex and demands great care and
expertise for specificity but provides subtype and mixed infection
information, in contrast to the serotyping assay. Detection of a
mixed infection by a type-specific PCR is relatively frequent and
should be interpreted with caution. In the nut shell, the type
specific PCR is a convenient method.
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