A simple spectrophotometric method for determination of cefaclor in
raw and dosage forms based on reaction with ninhydrin is developed,
optimized and validated. The purple color (Ruhemenn's purple)
resulted from the reaction was stabilized and measured at 560
nm.The method optimization was essential as many procedural
parameters influenced the accuracy of determination all variables
including the reagent concentration, heating time, reaction
temperature, color stability period, and cefaclor/ninhydrin ratio
were studied in order to optimize the reaction conditions. No
interference was observed from common pharmaceutical adjuvant. The
developed method is easy to use, accurate and highly cost-effective
for routine studies relative to HPLC and other techniques.
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