The first part of this investigation deals with induction of callus
and regeneration of haploid plants from anther cultures of rice. Z2
containing 2,4-D 0.5 mg/l+NAA 2.5 mg/l+KIN 0.5 mg/l induced highest
6.36% callus in BRRI dhan-29. Medium containing 4.0% sucrose gave
the highest 7.5% callus formation as well as highest green plants
regeneration. Pretreatment of cold shock is beneficial for callus
induction and regeneration of haploid plants. Chromosomes counting
was made from root tips and observed 69.0% haploids (n=12) in BRRI
dhan-29. In the second part effect of three basal media MS, N6 and
LS supplemented with four growth regulator treatments on the
response of induction of callus from three different sources of
explants like mature seed scutella, roots and immature embryos of
four rice genotypes was studied. The highest callus formation 88.8%
was recorded in Taipei-309 from mature seed scutella. Calli were
subcultured with tryptophan in MS+2,4-D(1.0 & 1.5 mg/l)+NAA 0.5
mg/l for somatic embryogenesis. Regeneration response of
embryogenic calli was tested on RM1, RM2 and RM3 and highest 79.5%
calli formed shoots in BRRI dhan-29 from immature embryos in RM3.
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