Electroporation Protocols - Preclinical and Clinical Gene Medicine (Hardcover, 2nd ed. 2014)

Electroporation gene therapy, or gene electrotransfer, has evolved greatly over the last few decades as a result of the remarkable progress in genetic sequencing, gene array analysis, gene cloning, gene expression detection, DNA manufacture and discovery and synthesis of siRNA. Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Electroporation Protocols: Preclinical and Clinical Gene Medicine, Second Edition aims to provide not only comprehensive coverage of the basic theory and practical application of electroporation siRNA therapy, gene therapy, and vaccine, but also elaborates on the most current views from the experts in this field, serving as an invaluable resource for investigators both in and outside of this field.

General

Imprint:	HumanaPress
Country of origin:	United States
Series:	Methods in Molecular Biology, 1121
Release date:	February 2014
First published:	2014
Editors:	Shulin Li • Jeffry Cutrera • Richard Heller • Justin Teissie
Dimensions:	254 x 178 x 24mm (L x W x T)
Format:	Hardcover
Pages:	349
Edition:	2nd ed. 2014
ISBN-13:	978-1-4614-9631-1
Categories:	Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics
LSN:	1-4614-9631-4
Barcode:	9781461496311

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