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MicroRNAs and the Immune System - Methods and Protocols (Hardcover, 2010 ed.)
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MicroRNAs and the Immune System - Methods and Protocols (Hardcover, 2010 ed.)
Series: Methods in Molecular Biology, 667
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In recent years, the critical role of microRNAs has been revealed
within the biology of cells that constitute the immune system. In
MicroRNAs and the Immune System: Methods and Protocols, expert
researchers explore the latest techniques for studying miRNA
expression, including the most up-to-date data on splinted ligation
and qRT-PCR assays, as well as high-throughput profiling through
cloning, deep sequencing, and microarrays. Chapters outline methods
to study miRNA functions in various cell types from a single cell
type level to entire model organisms, and present studies of miRNAs
in the context of viruses and the immune response. Tools are also
provided to help navigate bioinformatics databases on miRNAs and
their targets. Composed in the highly successful Methods in
Molecular Biology (TM) series format, each chapter contains a brief
introduction, step-by-step methods, a list of necessary materials,
and a Notes section which shares tips on troubleshooting and
avoiding known pitfalls. Contemporary and innovative, MicroRNAs and
the Immune System: Methods and Protocols is an essential handbook
for immunologists, biochemists, and molecular biologists.
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