Different aspects of micropropagation through meristem culture for
the production of virus indexed source plants, callus induction
followed by subsequent plant regeneration, in vitro tuberization
and field evaluation of the in vitro regenerated plants were
studied on four commercial cultivars of potato (Solanum tuberosum
L.) viz., Diamant, Cardinal, Shilbilati and Lalpakri. Rooted
plantlets of four potato cultivars obtained directly through
meristem culture and indirectly via callus phase were gradually
acclimatized and successfully established in the field. Visual
evaluation of the morphological traits of the meristem-derived
plants showed that all plants were normal and free from virus
diseases. Substantial yield increase was observed from
meristem-derived plants over their source plants. Somaclonal
variation among different plants derived from callus was observed.
The present investigation also addressed in vitro tuberization in
potato. The potato plants derived from microtubers of four
varieties under field condition was similar to look as that of
normal seed propagated plants.
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