This volume presents authoritative and cutting-edge methods and
protocols focusing on three tool boxes covering the increasingly
diverse methodologies used to image selected proteins and to
investigate their function by light and electron microscopy. The
first tool box includes the development of a wide range of
molecular and immunological probes to target specific proteins. The
second details the use of these probes for high resolution
fluorescence microscopy and the third focuses on applications for
transmission and scanning electron microscopy. Written in the
highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls thus ensuring successful results in the
further study of this vital field.
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