A biosensor is an integrated bioanalytical device that employs
biological elements, as analyte sensor, coupled to a transducer for
signal detection. Biosensor provides sensitive and selective
determination of various compounds of analytical relevance.
Recently, use of self-assembled monolayer (SAM) is gaining
attention as biosensor matrix. SAM provides a simple route to
functionalize electrode surfaces for biosensor fabrication. The
alarming rise in the rates of heart diseases due to abnormal levels
of cholesterol in blood has stimulated public concern about the
estimation of cholesterol level in desired sample. The present book
highlights the preparation, characterization and application of
some SAMs for immobilization of cholesterol oxidase, cholesterol
esterase & horseradish peroxidase for development of
cholesterol biosensor. The fabricated bioelectrodes were tested for
various parameters like pH, temperature, interferents etc. and have
been utilized for free and total cholesterol estimation in solution
& serum samples. The results indicate that the SAMs are likely
to provide useful matrices for the fabrication of commercial
cholesterol sensing bioelectrodes.
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