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Books > Science & Mathematics > Biology, life sciences > Microbiology (non-medical)
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Hydrocarbon and Lipid Microbiology Protocols - Genetic, Genomic and System Analyses of Pure Cultures (Hardcover, 1st ed. 2017)
Loot Price: R3,746
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Hydrocarbon and Lipid Microbiology Protocols - Genetic, Genomic and System Analyses of Pure Cultures (Hardcover, 1st ed. 2017)
Series: Springer Protocols Handbooks
Expected to ship within 12 - 19 working days
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This Volume presents a comprehensive series of generic protocols
for the genetic and genomic analysis of prokaryotic isolates.
Genetic methods for functional analyses employ the latest cloning
vectors, gene fusion methods and transposon mutagenesis systems, as
well as systems for introducing protease-cleavage sequences into
permissive sites in proteins under investigation. Genomic methods
described include protocols for transcriptomics, shotgun
proteomics, interactomics, metabolic profiling, and lipidomics.
Bioinformatic tools for genome annotation, transcriptome display
and the integration of transcriptomic data into genome-scale
metabolic reconstructions are described. Protocols for 13C-based
metabolic flux determinations and analysis of the hierarchical and
metabolic regulation of fluxes through pathways are included. The
Volume thus enables investigators to functionally analyse an
isolate over the entire cellular range spanning the gene, the
genome, the transcript repertoire, the proteome, the interactome,
the metabolic network with its nodes and their regulatory
hierarchies, and the metabolic fluxes and their physiological
controls. Hydrocarbon and Lipid Microbiology Protocols There are
tens of thousands of structurally different hydrocarbons,
hydrocarbon derivatives and lipids, and a wide array of these
molecules are required for cells to function. The global
hydrocarbon cycle, which is largely driven by microorganisms, has a
major impact on our environment and climate. Microbes are
responsible for cleaning up the environmental pollution caused by
the exploitation of hydrocarbon reservoirs and will also be pivotal
in reducing our reliance on fossil fuels by providing biofuels,
plastics and industrial chemicals. Gaining an understanding of the
relevant functions of the wide range of microbes that produce,
consume and modify hydrocarbons and related compounds will be key
to responding to these challenges. This comprehensive collection of
current and emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
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