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Hydrocarbon and Lipid Microbiology Protocols - Activities and Phenotypes (Hardcover, 1st ed. 2017)
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Hydrocarbon and Lipid Microbiology Protocols - Activities and Phenotypes (Hardcover, 1st ed. 2017)
Series: Springer Protocols Handbooks
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This Volume features protocols for investigating the hydrocarbon-
and lipid-specific activities of microbes. They include methods for
studying chemotaxis, the colonisation of hydrocarbon surfaces,
hydrocarbon uptake, respiration, nitrogen fixation, sulphate
reduction, membrane stabilisation through cis-trans isomerisation
of membrane fatty acids, and the production of biosurfactants and
biopolymers in response to the presence of hydrocarbons. A protocol
for studying the ability of microbes to control the concentration
of hydrocarbons in their aqueous environment is also described, and
phenotyping methods to reveal microbes' more general metabolic
activities are presented. Several protocols for investigating acid
production in connection with oil souring and biocorrosion by
microbes in oil well, oil transportation and storage settings are
presented. Lastly, protocols for measuring methanogenesis, as an
example of microbial hydrocarbon production, are described.<
Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of
thousands of structurally different hydrocarbons, hydrocarbon
derivatives and lipids, and a wide array of these molecules are
required for cells to function. The global hydrocarbon cycle, which
is largely driven by microorganisms, has a major impact on our
environment and climate. Microbes are responsible for cleaning up
the environmental pollution caused by the exploitation of
hydrocarbon reservoirs and will also be pivotal in reducing our
reliance on fossil fuels by providing biofuels, plastics and
industrial chemicals. Gaining an understanding of the relevant
functions of the wide range of microbes that produce, consume and
modify hydrocarbons and related compounds will be key to responding
to these challenges. This comprehensive collection of current and
emerging protocols will facilitate acquisition of this
understanding and exploitation of useful activities of such
microbes.
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