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Hydrocarbon and Lipid Microbiology Protocols - Genetic, Genomic and System Analyses of Communities (Hardcover, 1st ed. 2017)
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Hydrocarbon and Lipid Microbiology Protocols - Genetic, Genomic and System Analyses of Communities (Hardcover, 1st ed. 2017)
Series: Springer Protocols Handbooks
Expected to ship within 12 - 17 working days
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This Volume presents protocols for investigating the genetic,
metabolic and ecological potential and functional analysis of
microbial communities. Methods are described for the creation and
bioinformatic assessment of metagenomic and metatranscriptomic
libraries, and for metaproteomic analyses, which provide important
insights into the metabolic potential and interactions of community
members. These in turn lead to specific hypotheses concerning the
functional contributions of individual populations in the
community, which may be investigated by the stable isotope probing
approaches described in this Volume, making it possible to identify
those community members primarily responsible for particular
functions. Methods for the direct extraction of proteins from
environmental samples for sequencing and activity tests are
presented, providing a broad overview of prevailing metabolic
activities and of the types of microbe involved in them. Protocols
for the analysis of nutrient flow through microbial communities and
for the modelling of dynamic physiological interactions in
communities are also provided. Lastly, the book presents a protocol
for the quantitative assessment of permissiveness for the transfer
of conjugative plasmids, important agents of physiological change
and evolution in microbial communities. Hydrocarbon and Lipid
Microbiology ProtocolsThere are tens of thousands of structurally
different hydrocarbons, hydrocarbon derivatives and lipids, and a
wide array of these molecules are required for cells to function.
The global hydrocarbon cycle, which is largely driven by
microorganisms, has a major impact on our environment and climate.
Microbes are responsible for cleaning up the environmental
pollution caused by the exploitation of hydrocarbon reservoirs and
will also be pivotal in reducing our reliance on fossil fuels by
providing biofuels, plastics and industrial chemicals. Gaining an
understanding of the relevant functions of the wide range of
microbes that produce, consume and modify hydrocarbons and related
compounds will be key to responding to these challenges. This
comprehensive collection of current and emerging protocols will
facilitate acquisition of this understanding and exploitation of
useful activities of such microbes.
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