Quantitation of mRNA by Polymerase Chain Reaction - Nonradioactive PCR Methods (Paperback, Softcover reprint of the original 1st ed. 1995)

In this laboratory "cook-book," the authors provide a concise guide to PCR-based techniques to quantify nucleic acids in biological and clinical samples using exclusively nonradioactive detection methods, e.g. HPLC, biotin and digoxigenin based protocols. Each method presentation also includes sections on theory, reagents, standards, applicability, limitations, and trouble shooting. In addition to the protocols, the authors also provide the necessary information on: general aspects of nucleic acid quantitation; design of PCR standards; mRNA purification; cDNA synthesis; solution hybridization; DNA sequencing. This laboratory guide enables professionals as well as beginners to adopt easily quantitative PCR protocols into their own clinical or biomedical research.

General

Imprint:	Springer-Verlag
Country of origin:	Germany
Series:	Springer Lab Manuals
Release date:	December 2011
First published:	1995
Authors:	Thomas Koehler • Dirk Lassner • Anne-Katrin Rost • Barbara Thamm • Barbara Pustowoit • Harald Remke
Dimensions:	235 x 155 x 10mm (L x W x T)
Format:	Paperback
Pages:	166
Edition:	Softcover reprint of the original 1st ed. 1995
ISBN-13:	978-3-642-79714-9
Categories:	Books > Medicine > Pre-clinical medicine: basic sciences > Medical genetics Books > Science & Mathematics > Chemistry > Analytical chemistry > General Books > Science & Mathematics > Biology, life sciences > Biochemistry > General
LSN:	3-642-79714-8
Barcode:	9783642797149

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