How can these compounds be separated? Why was that method used?
These are the two basic questions often asked by students of
chromatography. HPLC: A Practical Guide provides the answers,
enabling the reader to grasp the concepts of the technique using
simple, representative chromatograms. Divided into six chapters,
this practical guide covers basic concepts of HPLC;
instrumentation; stationary phase materials; eluents; column
efficiency; and the influence of physical chemistry on separations.
Focusing on the basic considerations such as selection of
stationary phase and eluent, rather than specific applications,
sections on troubleshooting are also included. Uniquely, the
descriptions of chromatographic separations are based on solubility
using molecular properties, and solubility parameters are used to
analyse the selections of chromatographic mode and column.
Presenting the chemistry of liquid chromatography for undergraduate
students, this valuable practical guide will also be useful for
laboratory staff in industry and academia.
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