Development of imunoassays for haptens like steroids requires
conjugation of these apolar and low molecular weight molecules with
usually polar and larger proteins molecules for preparation of
desired immunogen and enzyme conjugate. Conjugation chemistries
employed in preparation of these immunoconjugates to a very large
extent define the assay parameters like specificity and
sensitivity. This book is aimed at critically examining the role of
the spacer or linker arms used in preparation of immunogens and
enzyme conjugates in hapten immunoassay, by compairing polarities,
chain length, rigidity and fexibilities of these linkers molecules.
The book is an attempt to bring together the various aspects of
conjugation chemistry and immunoassay devlopment of haptens in
general and would be specially useful for proffessionals and
researchers in the field of immunodiagnostics and combitorial
chemistry.
General
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