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Mammalian Artificial Chromosomes - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2004)
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Mammalian Artificial Chromosomes - Methods and Protocols (Paperback, Softcover reprint of the original 1st ed. 2004)
Series: Methods in Molecular Biology, 240
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In 1996, we organized a workshop, inter alia, at the National
Research Co- cil in Milan under the generous sponsorship of the
European Science Foun- tion. On that occasion, a small group of
investigators convened from many countries and presented early
evidence of the possibility of assembling basic units of mammalian
chromosomes into artificial constructs (or, indeed, red- ing the
relevant components to more manageable dimensions and defined c-
stitution). Progress in the following years has been slow but
steady. Many scientists who took part in the workshop have since
been engaged in active and prod- tive research. It goes to the
credit of Humana Press to have realized the need for a book on
artificial chromosomes that aims to provide better tools to all
scientists committed to this field who are confronted with very
difficult tech- cal problems. We have strived to cover in Mammalian
Artificial Chromosomes: Methods and Protocols all relevant areas of
artificial chromosome research, from basic genetics to daring
attempts to build new tools for genetic therapy. We are of course
grateful to the authors who have accepted the task of describing
the technical steps and pitfalls that can be encountered in their
research. Rarely has a very delicate methodology been presented
with such meticulous care. We have been helped in this enterprise
by the excellent librarian of the LITA Institute in Segrate, Italy,
Ms. Claudia Piergigli, whom we thank warmly. Ms.
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