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Plant Phosphoproteomics - Methods and Protocols (Hardcover, 2015 ed.)
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Plant Phosphoproteomics - Methods and Protocols (Hardcover, 2015 ed.)
Series: Methods in Molecular Biology, 1306
Expected to ship within 12 - 17 working days
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This detailed volume addresses recent developments in
phosphoproteomic techniques with a particular focus on the plant
system. Over the recent decades, proteomic methods were refined to
study the significance and dynamics of protein phosphorylation in
various biological contexts. However, working with plant tissue
imposes particular challenges to the biologist which are attributed
to the rigid cell wall making protein extraction more difficult,
the skewed protein abundance with Rubisco as a highly abundant
protein and a large central vacuole leading to low protein yield
and increased degradative enzyme activity. The methodologies in
this book seek to move beyond these issues. Written for the Methods
in Molecular Biology series, chapters include introductions to
their respective topics, lists of the necessary materials and
reagents, step-by-step, readily reproducible laboratory protocols
and tips on troubleshooting and avoiding known pitfalls. Practical
and authoritative, Plant Phosphoproteomics: Methods and Protocols
serves as an ideal reference for researchers investigating this
vital area of plant science.
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